Control Mechanism of Phasic and Tonic <alpha> -Motoneurons Innervating Fast and Slow Muscles in the Chicken Spinal Cord

鸡脊髓中快肌和慢肌运动神经元的阶段性和强直性<α>运动神经元的控制机制

• 批准号: 60570027
• 负责人: ATSUMI Saoko
• 金额: $ 1.22万
• 依托单位: Yamanashi Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570027/
• 关键词: Phasic motoneuron; Tonic motoneuron; Substance P; Serotonin; Enkephalin; Acetylcholinesterase; <alpha> -Spectrin

In order to obtain a clue to know the control mechanism of phasic and tonic <alpha> -motoneurons innervating fast and slow muscles, a series of studies was carried out and following results were obtained.1. <alpha> -Motoneurons received inputs from substance P (SP), serotonin (5-HT) and enkephalin (Enk)-containing presynaptic boutons. The SP-immunoreactive presynaptic bouton contained spherical dense-cored vesicles and clear vesicles, 5-HT-immunoreactive bouton elongated dense cored vesicles and flattened clear vesicles, and Enk-immunoreactive bouton spherical dense cored vesicles and clear vesicles of mixed types.2. Acetylcholinesterase activity was observed in the synaptic cleft of the two types of presynaptic boutons: (1) S type bouton; (2) boutons containing spherical dense cored vesicles and spherical clear vesicles.3. <alpha> -Spectrin was purified from chicken red blood cell ghosts and specific antibody against <alpha> -spectrin was raised in rabbits. Using the specific antibody … More , the localization of <alpha> -spectrin was studied immunocytochemically in the chicken and monkey ventral horns. <alpha> -Spectrin-like immunoreactivity was observed in cortical cytoplasm of the soma, dendrites and axon of large ventral horn cells. The postsynaptic density was particularly intensely immunostained. The immunostaining was also associated with neurotubules in the axon and dendrites.4. The SP-like immunoreactive structures were studied in the laminae I and II of the chicken dorsal horn. Two kinds of immunoreactive boutons were observed: (1) the immunoreactive presynaptic boutons showing approximately circular profile which synapsed with unstained dendrites; (2) the synaptic glomerulus in which a few immunoreactive axon terminals were surrounded by several axons and dendrites. In the latter, central axon terminals constituted presynaptic components in most synapses but in a few postsynaptic components. Septate junction-like structures were observed between individual central axon terminals.5. Quantitative analyses of the synaptology of phasic and tonic <alpha> -motoneurons were performed. The ratios of the number of Gf-type boutons (PLD>ALD) and S type boutons (PLD<ALD) to the total number of boutons were significant. Concerning the covering ratio, Gf, Gs and total covering ratios were significantly larger in PLD than in ALD. Less

为了了解支配快肌和慢肌的阶段性和强直性α-运动神经元的控制机制，进行了一系列的研究，得到了以下结果： 1．含有 P (SP)、血清素 (5-HT) 和脑啡肽 (Enk) 的突触前按钮 SP 免疫反应性突触前按钮包含球形。致密核心囊泡和透明囊泡，5-HT免疫反应拉长致密核心囊泡和扁平透明囊泡，Enk免疫反应球形致密核心囊泡和透明囊泡的混合类型。 2．突触前波顿的两种类型：（1）S型(2)含有球形致密核心囊泡和球形透明囊泡的囊泡。3.从鸡红细胞血影中纯化<α>-血影蛋白，并使用该特异性抗体在兔子中产生针对<α>-血影蛋白的特异性抗体。此外，还对鸡和猴腹角中α-血影蛋白的定位进行了免疫细胞化学研究，观察到了α-血影蛋白样免疫反应性。在大腹角细胞的胞体、树突和轴突的皮质细胞质中，突触后密度特别强烈，免疫染色也与轴突和树突中的神经管有关。4．观察到鸡背角 I 和 II 两种免疫反应性小结：（1）免疫反应性突触前小结呈现近似圆形轮廓。突触与未染色的树突；（2）突触肾小球，其中一些免疫反应性轴突末端被几个轴突和树突包围。在后者中，中央轴突末端在大多数突触中构成突触前成分，但在少数突触后成分中。观察各个中央轴突末端之间的结构。5．阶段性和强直性突触的定量分析。进行了α-运动神经元的检测，Gf型扣环(PLD>ALD)和S型扣环(PLD<ALD)的数量与覆盖率、Gf、Gs和总扣环数量的比率是显着的。 PLD 中的总覆盖率明显大于 ALD 中的总覆盖率。

项目成果

E. Ozawa, K. Saito, Y. Hagiwara, A. Shoji, K. Saito and S. Atsumi: In " Molecular Biology of Muscle Development" ed. by B. W. Ginard, D. Fishman and C. Emersson Alan R. Liss Inc., New York. Inorganic iron ion supports muscle growth and differentiation as

E. Ozawa、K. Saito、Y. Hagiwara、A. Shoji、K. Saito 和 S. Atsumi：《肌肉发育的分子生物学》编辑。

T.Shimo-Oka;S.Atsumi: Journal of Neurocytology. 15. 715-723 (1986)

T.Shimo-Oka；S.Atsumi：神经细胞学杂志。

S. Atsumi and H. Sakamoto: "Enkephalin-like immunoreactive axon terminals make synapses with <alpha> - motoneurons in the chicken." Brain Research. (1987)

S. Atsumi 和 H. Sakamoto：“鸡体内的脑啡肽样免疫反应性轴突末端与 <α> - 运动神经元形成突触。”

Y.Hagiwara;S.Atsumi;E.Ozawa: Journal of Pharmacobio-Dynamics. 8. 311-319 (1985)

Y.Hagiwara；S.Atsumi；E.Ozawa：药物生物动力学杂志。

H. Sakamoto, K. Ohsato and S. Atsumi: "Acetylcholinesterase activity at the synapses of presynaptic boutons with presumed <alpha> -motoneurons in chicken ventral horn. Light- and electronmicroscopic studies." Histochemistry. 83. 291-298 (1985)

H. Sakamoto、K. Ohsato 和 S. Atsumi：“突触前突触处的乙酰胆碱酯酶活性与鸡腹角中假定的 <α> 运动神经元。光学和电子显微镜研究。”