Control Mechanism of Phasic and Tonic <alpha> -Motoneurons Innervating Fast and Slow Muscles in the Chicken Spinal Cord

鸡脊髓中快肌和慢肌运动神经元的阶段性和强直性<α>运动神经元的控制机制

• 批准号: 60570027
• 负责人: ATSUMI Saoko
• 金额: $ 1.22万
• 依托单位: Yamanashi Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570027/
• 关键词: Phasic motoneuron; Tonic motoneuron; Substance P; Serotonin; Enkephalin; Acetylcholinesterase; <alpha> -Spectrin

In order to obtain a clue to know the control mechanism of phasic and tonic <alpha> -motoneurons innervating fast and slow muscles, a series of studies was carried out and following results were obtained.1. <alpha> -Motoneurons received inputs from substance P (SP), serotonin (5-HT) and enkephalin (Enk)-containing presynaptic boutons. The SP-immunoreactive presynaptic bouton contained spherical dense-cored vesicles and clear vesicles, 5-HT-immunoreactive bouton elongated dense cored vesicles and flattened clear vesicles, and Enk-immunoreactive bouton spherical dense cored vesicles and clear vesicles of mixed types.2. Acetylcholinesterase activity was observed in the synaptic cleft of the two types of presynaptic boutons: (1) S type bouton; (2) boutons containing spherical dense cored vesicles and spherical clear vesicles.3. <alpha> -Spectrin was purified from chicken red blood cell ghosts and specific antibody against <alpha> -spectrin was raised in rabbits. Using the specific antibody … More , the localization of <alpha> -spectrin was studied immunocytochemically in the chicken and monkey ventral horns. <alpha> -Spectrin-like immunoreactivity was observed in cortical cytoplasm of the soma, dendrites and axon of large ventral horn cells. The postsynaptic density was particularly intensely immunostained. The immunostaining was also associated with neurotubules in the axon and dendrites.4. The SP-like immunoreactive structures were studied in the laminae I and II of the chicken dorsal horn. Two kinds of immunoreactive boutons were observed: (1) the immunoreactive presynaptic boutons showing approximately circular profile which synapsed with unstained dendrites; (2) the synaptic glomerulus in which a few immunoreactive axon terminals were surrounded by several axons and dendrites. In the latter, central axon terminals constituted presynaptic components in most synapses but in a few postsynaptic components. Septate junction-like structures were observed between individual central axon terminals.5. Quantitative analyses of the synaptology of phasic and tonic <alpha> -motoneurons were performed. The ratios of the number of Gf-type boutons (PLD>ALD) and S type boutons (PLD<ALD) to the total number of boutons were significant. Concerning the covering ratio, Gf, Gs and total covering ratios were significantly larger in PLD than in ALD. Less

为了获得一个线索，了解阶段性和补品<Alpha> - 肌神经元的控制机制，以快速和缓慢的肌肉支配，进行了一系列研究，并获得了以下结果。1。 <Alpha> -Motoneurons从P（SP），5-羟色胺（5-HT）和Enkephalin（ENK）（ENK）（含有突触前的船东）中收到输入。 SP免疫反应性突触前的Bouton含有球形密集的蔬菜和透明的蔬菜，5-HT-免疫反应性的BOUTON细长浓密的浓密的内核蔬菜以及扁平的透明蔬菜，以及Enk-rumunoreactive bouton-rumunoreactive bouton bouton bouton式的浓密的钢芯蔬菜和透明的蔬菜。在两种类型的突触前胸子的突触裂缝中观察到乙酰胆碱酯酶活性：（1）S型Bouton； （2）包含球形密集的蔬菜和球形透明蔬菜的胸衣3。 <Alpha> -spectrin是从鸡红细胞幽灵中纯化的，对<alpha> -spectrin的特异性抗体在兔子中提出。使用特定的抗体……更多，在鸡肉和猴子腹角中对<Alpha> - 谱蛋白的定位进行了研究。 <Alpha> - 光谱样免疫反应性在大型腹侧角细胞的躯体，树突和轴突的皮质细胞质中观察到。突触后密度特别紧密地免疫染色。免疫染色也与轴突和树突中的神经管有关。4。在鸡背喇叭的Laminae I和II中研究了SP状的免疫反应性结构。观察到两种免疫反应性胸子：（1）免疫反应性突触前胸子，显示出与未固定的树突突触的大约圆形轮廓； （2）突触肾小球，其中一些免疫反应性轴突末端被几个轴突和树突包围。在后者中，中央轴突末端在大多数突触中构成突触前成分，但在一些突触后成分中。在单个中央轴突端子之间观察到分隔连接样结构。5。进行了突触和补品<alpha> - 微神经元的定量分析。 GF型胸子（PLD> ALD）和S型式式式式武物（PLD <ALD）与船座总数的比率很大。关于覆盖率，GF，GS和总覆盖率在PLD中明显大于ALD。较少的

项目成果

E. Ozawa, K. Saito, Y. Hagiwara, A. Shoji, K. Saito and S. Atsumi: In " Molecular Biology of Muscle Development" ed. by B. W. Ginard, D. Fishman and C. Emersson Alan R. Liss Inc., New York. Inorganic iron ion supports muscle growth and differentiation as

E. Ozawa、K. Saito、Y. Hagiwara、A. Shoji、K. Saito 和 S. Atsumi：《肌肉发育的分子生物学》编辑。

T.Shimo-Oka;S.Atsumi: Journal of Neurocytology. 15. 715-723 (1986)

T.Shimo-Oka；S.Atsumi：神经细胞学杂志。

S. Atsumi and H. Sakamoto: "Enkephalin-like immunoreactive axon terminals make synapses with <alpha> - motoneurons in the chicken." Brain Research. (1987)

S. Atsumi 和 H. Sakamoto：“鸡体内的脑啡肽样免疫反应性轴突末端与 <α> - 运动神经元形成突触。”

Y.Hagiwara;S.Atsumi;E.Ozawa: Journal of Pharmacobio-Dynamics. 8. 311-319 (1985)

Y.Hagiwara；S.Atsumi；E.Ozawa：药物生物动力学杂志。

H. Sakamoto, K. Ohsato and S. Atsumi: "Acetylcholinesterase activity at the synapses of presynaptic boutons with presumed <alpha> -motoneurons in chicken ventral horn. Light- and electronmicroscopic studies." Histochemistry. 83. 291-298 (1985)

H. Sakamoto、K. Ohsato 和 S. Atsumi：“突触前突触处的乙酰胆碱酯酶活性与鸡腹角中假定的 <α> 运动神经元。光学和电子显微镜研究。”