Structure and function of the transcription factor AREC3

转录因子AREC3的结构和功能

基本信息

批准号：
07670152
负责人：
KAWAKAMI Kiyoshi
金额：
$ 1.66万
依托单位：
Jichi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670152/
关键词：
homeobox gene transcription factor development of retina early development neuronal cells Six family gene immunostaining in situ hybridization Sixファミリー遺伝子ホメオボックス蛋白質 sixファミリー網膜 cDNA 筋分化

项目摘要

1. Three species of cDNA encoding AREC3/Six4 protein has been cloned from mouse skeletal muscle cDNA library. Sequence analysis revealed that the AREC3 is a member of new gene family of Six which has homeodomain and sixdomain as conserved domains. Both of the domains are necessary for specific DNA binding, and potential transactivation domain resides in the C terminal portion. During the differentiation of myoblast cell line C2C12, the productio of AREC3 protein is induced in the cytoplasm. During postnatal retina formation in rat, the expression pattern of the gene product varies dramatically. These observations suggest that the gene is involved in development and differentiation process.2. Other members of Six family genes, Six2, Six3 and Six5 cDNAs were isolated from mouse retina cDNA library. These genes were shown to be expressed in retina by in situ hybridization. Homeodomain and sixdomain function as a specific DNA binding domain, and the DNA binding specificity was conserved am … More ong Six2, Six4 and Six5.3. The distribution of AREC3 protein was analyzed by immunohistochemistry in newborn the rat retina and in the adult rat brain. In PND (postnatal day)1, the AREC3 resided in the nucleus of ganglion cells, in PND4, in addition to PND1, the protein was expressed in the inner nuclear layr and in PND7, the expression was observed in outer cells of the inner nuclear layr. In PND13, the expression was moved from the nucleus to the cytoplasm in ganglion cells, and the protein was expressed in outer segment and inner segment. After PND20, no distribution in the nucleus was observed. In rat brain, AREC3 protein was observed in the cell nucleus in hippocampus and in the piriform cortex and the mRNA was observed in the cytoplasm.4. In the mouse embryo, the AREC3 protein was detected in the nucleus of neuronal cells from stage E9.5. The production peaks at E10.5 to E11.5 and then gradually declined to undetectable level at E14.5These results indicates that the AREC3 plays an important role in differentiation and development of neuron and retina. Less

1.从小鼠骨骼肌cDNA文库中克隆了三种编码AREC3/Six4蛋白的cDNA，序列分析表明AREC3是Six新基因家族的成员，该家族具有同源结构域和six结构域这两个结构域。特异性 DNA 结合所必需的，并且潜在的反式激活结构域位于 C 末端部分。在成肌细胞系 C2C12 的分化过程中，AREC3 蛋白的产生被诱导。在大鼠出生后视网膜形成过程中，该基因产物的表达模式变化很大。2．分离了6个家族基因的其他成员Six2、Six3和Six5 cDNA。来自小鼠视网膜 cDNA 文库的这些基因通过原位杂交显示在视网膜中表达，并且 Sixdomain 充当特定的 DNA 结合域，并且 DNA 结合特异性在 Six2、Six4 和 Six4 中保持不变。 Six5.3.通过免疫组织化学分析新生大鼠视网膜和成年大鼠大脑中AREC3蛋白的分布。在PND（出生后）1中，除了PND1之外，AREC3还存在于神经节细胞的细胞核中。，该蛋白在内核层表达，在PND7中，在内核层的外细胞中观察到表达，在PND13中，表达从细胞核转移到细胞质。 PND20后，在神经节细胞中未观察到AREC3蛋白在细胞核中的分布，且在外节和内节中没有观察到AREC3蛋白的分布，并且在梨状皮层中观察到AREC3蛋白的mRNA表达。 4.在小鼠胚胎中，E9.5期神经元细胞核中检测到AREC3蛋白，在E10.5至E11.5时产量达到峰值，随后逐渐下降。在E14.5处检测不到的水平这些结果表明AREC3在神经元和视网膜的分化和发育中发挥着重要作用。