Structure and function of the transcription factor AREC3

转录因子AREC3的结构和功能

基本信息

批准号：
07670152
负责人：
KAWAKAMI Kiyoshi
金额：
$ 1.66万
依托单位：
Jichi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670152/
关键词：
homeobox gene transcription factor development of retina early development neuronal cells Six family gene immunostaining in situ hybridization Sixファミリー遺伝子ホメオボックス蛋白質 sixファミリー網膜 cDNA 筋分化

项目摘要

1. Three species of cDNA encoding AREC3/Six4 protein has been cloned from mouse skeletal muscle cDNA library. Sequence analysis revealed that the AREC3 is a member of new gene family of Six which has homeodomain and sixdomain as conserved domains. Both of the domains are necessary for specific DNA binding, and potential transactivation domain resides in the C terminal portion. During the differentiation of myoblast cell line C2C12, the productio of AREC3 protein is induced in the cytoplasm. During postnatal retina formation in rat, the expression pattern of the gene product varies dramatically. These observations suggest that the gene is involved in development and differentiation process.2. Other members of Six family genes, Six2, Six3 and Six5 cDNAs were isolated from mouse retina cDNA library. These genes were shown to be expressed in retina by in situ hybridization. Homeodomain and sixdomain function as a specific DNA binding domain, and the DNA binding specificity was conserved am … More ong Six2, Six4 and Six5.3. The distribution of AREC3 protein was analyzed by immunohistochemistry in newborn the rat retina and in the adult rat brain. In PND (postnatal day)1, the AREC3 resided in the nucleus of ganglion cells, in PND4, in addition to PND1, the protein was expressed in the inner nuclear layr and in PND7, the expression was observed in outer cells of the inner nuclear layr. In PND13, the expression was moved from the nucleus to the cytoplasm in ganglion cells, and the protein was expressed in outer segment and inner segment. After PND20, no distribution in the nucleus was observed. In rat brain, AREC3 protein was observed in the cell nucleus in hippocampus and in the piriform cortex and the mRNA was observed in the cytoplasm.4. In the mouse embryo, the AREC3 protein was detected in the nucleus of neuronal cells from stage E9.5. The production peaks at E10.5 to E11.5 and then gradually declined to undetectable level at E14.5These results indicates that the AREC3 plays an important role in differentiation and development of neuron and retina. Less

1。编码AREC3/SIX4蛋白的三种cDNA已从小鼠骨骼肌cDNA文库中克隆。序列分析表明，AREC3是六个中的新基因家族的成员，该家族具有谱系域和六域作为配置域的成员。这两个结构域对于特定的DNA结合都是必需的，并且潜在的反式激活结构域位于C末端部分。在成肌细胞细胞系C2C12的分化过程中，AREC3蛋白的产物在细胞质中诱导。在大鼠出生后视网膜形成期间，基因产物的表达模式各异。这些观察结果表明该基因参与了开发和分化过程。2。从小鼠视网膜cDNA库中分离出六个家庭基因的其他成员，分别是六个家庭基因。这些基因通过原位杂交在视网膜中表达。同源域和六域作为特定的DNA结合结构域的功能，而DNA结合特异性是AM…更多ONG SIX2，SIX4和SIX5.3。通过新生儿大鼠视网膜和成年大鼠大脑中的免疫组织化学分析了AREC3蛋白的分布。在PND（产后）1中，AREC3驻留在神经节细胞的核us中，在PND4中，除了PND1外，该蛋白在内部的核Layr中表达，在PND7中，在内部核Layr的外细胞中观察到表达。在PND13中，在神经节细胞中，表达式从核转移到细胞质，并在外部段和内部段中表达蛋白质。 PND20后，未观察到核分布。在大鼠脑中，在海马和梨状皮层中的细胞核中观察到AREC3蛋白，并且在Cytoplasm.4中观察到mRNA。在小鼠胚胎中，从E9.5期中检测到神经元细胞的核中AREC3蛋白。生产在E10.5至E11.5处峰值，然后逐渐降低到E14.5的无法检测到的水平，这些结果表明AREC3在神经元和视网膜的分化和发展中起着重要作用。较少的