Development of Nitroarene Analyzer

硝基芳烃分析仪的研制

基本信息

批准号：
06557121
负责人：
HAYAKAWA Kazuichi
金额：
$ 3.58万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

(1) Both the nitroarene analyzer and the Ames test are necessary in the study on mutagenic nitroarenes. For the simultaneous use of these two methods, several solvent systems were tested to extract 1,3-dinitropyrene (1,3-DNP), 1,6-DNP,1,8-DNP and 1-nitropyrene (1-NP) as well as directacting mutagenic activity from airborne particulates. Benzene-ethanol, acetonitrile, ethanol and methanol gave the high efficiencies. Considering toxicities, ethanol seemed to be the best solvent.(2) Although the nitroarene analyzer determined trace levels of 1,3-, 1,6-and 1,8-DNPs and 1-NP in extracts from a few mg of airborne particulates, the reduction of nitroarenes to corresponding aminoarenes was required before injection. To remove this tedious treatment, a reducer column packed with zinc/glass beads was on-line introduced into the system. Considering the better resolution of aminoarenes, both the reducer column and a switching valve equipped with a heart-cutting loop were introduced just before the … More ODS separation column. This system could determine the above four compounds in extracts from airborne particulatates without pre-column reduction.(3) In the method, not only nitroarenes but also polycyclic aromatic hydrocarbons (PAHs) were quantitatively extracted from airborne partculates. To determine them simultaneously, both the nitroarene analyzer and a conventional high-performance liquid chromatograph with a fluorescence detector were combined using a switching valve. After the reduced sample solution was injected, four nitroarenes and eight PAHs were separately determined in the two different lines in 1h with high sensitivities.(4) Several mutagenic nitroarenes such as 2-, 4-NPs, 2-nitrofluoranthene and 6-nitrochrycene were formed in air in the presence of NOx. To determine these compounds, a reducer column packed with zinc/glass beads and two ODS columns, one for the clean-up and the other for the concentration, were introduced into the nitroarene analyzer. By the system, the above four compounds were detected in air. The fact that their ratios to 1,3-, 1,6-, 1,8-DNPs and 1-NP in rural air were larger than those in urban air suggested the formation of those compounds during the transportation. However, their mutagenic contributions were not large. Less

（1）在有关诱变硝化碳酸盐的研究中，硝化分析仪和AMES测试都是必要的。为了简单地使用这两种方法，测试了几个溶剂系统以提取1,3-二硝基林（1,3-DNP），1,6-DNP，1,8-DNP和1-硝基吡喃（1-NP），并从空气中的成分中引导诱变活性。苯 - 乙醇，乙腈，乙醇和甲醇的效率很高。考虑到毒性，乙醇似乎是最好的解决方案。（2）尽管硝基苯乙烯分析仪确定了从几毫克的空气寄生成分中的1,3-，1,6和1,8-DNP和1-NP的痕量水平，但在注射之前需要减少硝基含量的硝酸含量。为了消除这种乏味的处理，将带有锌/玻璃珠的还原柱在线引入系统。考虑到更好的分辨率分辨率分辨率，在…更多ODS分离柱之前，都引入了还引入了带有心脏切开环的开关阀。该系统可以确定来自空气传播的特定物质提取物中的上述四种化合物，而无需列前还原。（3）在该方法中，不仅从硝基苯甲酸酯，而且还从空气中的部分中定量提取了多环芳烃（PAHS）。为了简单地确定它们，使用开关阀合并了硝化分析仪和带有荧光检测器的常规高性能液相色谱仪。注入还原样的样品溶液后，在1H中分别在1H中分别确定了两种不同的线，具有高灵敏度。（4）在诺克斯（Nox）的情况下，在空气中形成了几种诱变的硝化碳，例如2-，4-NP，2-硝基氟氨基和6-硝基烯丙基烯。为了确定这些化合物，将带有锌/玻璃珠和两个ODS柱的还原柱引入了硝基苯enen分析仪中。通过系统，在空气中检测到以上四种化合物。它们的比率与1,3-，1,6-，1,8-DNP和1-NP的粗糙空气比城市空气的比率大，这一事实表明在运输过程中形成了这些化合物。但是，它们的诱变贡献并不大。较少的