Development of X-ray fluorescence analysis of light elements in biological samples --- application to the study of Alzheimer disease

生物样品中轻元素X射线荧光分析的进展——在阿尔茨海默病研究中的应用

• 批准号: 06554034
• 负责人: NAKAI Izumi
• 金额: $ 4.03万
• 依托单位: Science University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06554034/
• 关键词: Aluminum; synchrotron radiation; X-ray fluorescence analysis; Alzheimer disease; Total reflection X-ray fluorescence analysis; biological trace element; trace element analysis; biopsy sample; アルツハイマー; 臨床試料; 蛍光X線; 微量元素; 生体試料

We have constructed a new X-ray fluorescence chamber for total reflection analysis and two-dimensional X-ray fluoresce analysis. This instrument is suitable for trace element analysis of biological samples such as biopsy samples (TR-XRF) and cancer tissues with complex structure (XRF imaging). X-rays from Synchrotron radiation and conventional X-ray tube can be used as the X-ray source. We have used this instrument to determine Al level in biological samples as given below. We have also determined the Zen and Cu levels from biopsy samples.Synchrotron radiation Induced total reflection X-ray fluorescence analysis of Al in biological samples was developed through examinations of sample preparation technique, internal standard and measurement conditions. The samples were standard solutions of Al and organs of Al administrated rat. The sample was heated in a current of oxygen gas and decomposed with concentrated nitric acid and hydrogen peroxide. Cr was chosen as an internal standard after consideration of its energy, coexisting elements in the sample and simplicity of the sample preparation. It was possible to determine Al of 10ng range using 7.25keV X-ray in air.

我们建造了一个新的X射线荧光室，用于全反射分析和二维X射线荧光分析。该仪器适用于活检样品（TR-XRF）和结构复杂的癌组织（XRF成像）等生物样品的微量元素分析。来自同步加速器辐射的X射线和传统X射线管可以用作X射线源。我们使用该仪器测定生物样品中的铝含量，如下所示。我们还测定了活检样品中Zen和Cu的含量。通过对样品制备技术、内标和测量条件的研究，开发了生物样品中Al的同步辐射诱导全反射X射线荧光分析方法。样品为铝标准溶液和铝给药大鼠器官。将样品在氧气流中加热并用浓硝酸和过氧化氢分解。考虑到其能量、样品中的共存元素以及样品制备的简单性，选择 Cr 作为内标。在空气中使用 7.25keV X 射线可以测定 10ng 范围内的 Al。

项目成果

S. Homma: "Site-specific distribution of copper, selenium, and zinc in human kidney by synchrotron radiation induced X-ray fluorescence"Nucl. Instr. And Meth. B. 103. 229-232 (1995)

S. Homma：“通过同步辐射诱导 X 射线荧光，铜、硒和锌在人肾中的位点特异性分布”Nucl。

N. Ohta: "Determination of trace amounts of Al in biological samples by Synchrotron radiation-induced total-reflection X-ray fluorescence analysis (in Japanese with English Abstract)"28. 207-216 (1997)

N. Ohta：“通过同步加速器辐射诱导的全反射 X 射线荧光分析测定生物样品中的痕量铝（日文，英文摘要）”28。

C. Numako: "XAFS studies of iron in the teeth of chiton, Acanthopleura Japonica."J. de Physique. IV C2. 615-616 (1997)

C. Numako：“对石鳖 (Acanthopleura Japonica) 牙齿中铁的 XAFS 研究。”J.

S. Homma-Takeda: "Direct detection of mercury-bound metalloproteins (metallo-thionein and Cu, Zn-Superoxide dismutase) using a combination of gel electrophoresis and one dimensional synchrotron radiation X-ray fluorescence analysis."Anal. Lett.. 29. 601-6

S. Homma-Takeda：“结合凝胶电泳和一维同步辐射 X 射线荧光分析直接检测汞结合金属蛋白（金属硫蛋白和铜、锌超氧化物歧化酶）。”

近藤奈穂子: "放射光全反射蛍光X線分析による生検試料中のCu,Znの定量"分析化学. 46. 957-964 (1997)

Naoko Kondo：“通过同步加速器全内反射荧光 X 射线分析对活检样品中的 Cu 和 Zn 进行定量”《分析化学》46. 957-964 (1997)。