SR-X-ray fluorescence analysis of arsenic poisoning biopsy samples

砷中毒活检样本的 SR-X 射线荧光分析

• 批准号: 11440219
• 负责人: NAKAI Izumi
• 金额: $ 7.04万
• 依托单位: Faculty of Science, Science Univ.of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11440219/
• 关键词: arsenic poisoning; X-ray fluorescence analysis; two dimensional analysis; X-ray micro beam; XAFS; synchrotron radiation; chemical state analysis; biopsy samples; 放射光蛍光X線分析; 毛髪断面; 有機ヒ素; 無機ヒ素; As K-XANES

Synchrotron radiation induced X-ray fluorescence (SR-XRF) analysis was applied to reveal arsenic level in human hair and skin. The samples are obtained from patients of acute and chronic arsenic poisonings. The former specimens were collected from victim of arsenic curry murder occurred at Wakayama city and the latter were from China, where serious arsenic environmental problem exists and chronic poisoning is generated in a few hundred thousands person scale. The distribution of arsenic along the length of a hair was measured by one-dimensional SR-XRF analysis and that in the vertical section was measured by two-dimensional analysis utilizing X-ray microbeam in 5 μm size. The skin samples obtained by biopsy was also examined. Chemical state of arsenic is strongly related with toxicity and therefore it is another important subject of the present study.One dimensional analysis of hairs from victims of arsenic poisoning show that presence of a clear peak of arsenic and high arsenic level … More continued for 1cm length (about two weeks). On the other hand, the results for the samples of chronic poisoning show monotonous distribution of arsenic. Two dimensional analysis of hair section has revealed high accumulation of arsenic at outer rim of the hair whereas zinc and sulfur was uniformly distributed. The distribution of copper was similar to that of arsenic. It is found for the first time that the accumulation of arsenic does happen in the skin from patients of chronic arsenic poisoning. The high arsenic level was observed at a region between corium and epidermis of the skin. XANES analysis of arsenic in the hair of acute poisoning has revealed that arsenic exists in the form of inorganic trivalent state. This result suggests that the arsenic was not metabolized in the acute poisoning. The present study clearly revealed the accumulation mechanism of arsenic in both acute and chronic arsenic poisonings. This study also demonstrated that SR-XRF is a powerful nondestructive technique to reveal distribution and chemical form of trace arsenic in biopsy samples. Less

应用同步辐射诱导X射线荧光（SR-XRF）分析来揭示人类头发和皮肤中的砷含量。这些样本是从急性和慢性砷中毒患者身上采集的。和歌山市的砷污染严重，砷污染严重，造成数十万人慢性中毒。通过一维 SR-XRF 分析测量，并通过利用 5 μm 大小的 X 射线微束的二维分析强烈测量砷的化学状态。毒性，因此它是本研究的另一个重要课题。对砷中毒受害者的头发进行一维分析表明，存在明显的砷峰和高砷水平……更多持续1厘米长度（约2厘米）另一方面，慢性中毒样品的结果显示砷的分布单调，头发切片的二维分析显示砷在头发的外缘大量积累，而锌和硫的分布均匀。铜的含量与砷的含量相似，首次发现慢性砷中毒患者皮肤中砷的积累，砷含量很高。对急性中毒头发中真皮和表皮之间区域的砷进行分析，结果表明砷在急性中毒时并未被代谢。清楚地揭示了急性和慢性砷中毒中砷的积累机制。这项研究还证明了 SR-XRF 是揭示分布和化学形态的强大无损技术。活检样品中痕量砷含量较少。

项目成果

Y.Terada et al.: "Development and application of multi-purpose X-ray Fluorescence analyzer using synchrotron and conventional X-ray sources"X-ray Spectrom.. 28. 461-463 (1999)

Y.Terada 等人：“使用同步加速器和常规 X 射线源的多用途 X 射线荧光分析仪的开发和应用”X 射线光谱.. 28. 461-463 (1999)

Y.Terada: "Development and application of multi-purpose X-ray fluorescence analyzer using synchrotron and conventional X-ray sources"X-ray Spectrom.. 28. 461-463 (1999)

Y.Terada：“使用同步加速器和常规X射线源的多用途X射线荧光分析仪的开发和应用”X射线光谱.. 28. 461-463 (1999)

Y.Terada, et.al.: "Development and application of multi-purpose X-ray Fluorescence analyzer using synchrotron and conventional X-ray sources"X-ray Spectrom. 28. 461-463 (1999)

Y.Terada 等人：“使用同步加速器和常规 X 射线源的多用途 X 射线荧光分析仪的开发和应用”X 射线光谱。