SR-X-ray fluorescence analysis of arsenic poisoning biopsy samples

砷中毒活检样本的 SR-X 射线荧光分析

• 批准号: 11440219
• 负责人: NAKAI Izumi
• 金额: $ 7.04万
• 依托单位: Faculty of Science, Science Univ.of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11440219/
• 关键词: arsenic poisoning; X-ray fluorescence analysis; two dimensional analysis; X-ray micro beam; XAFS; synchrotron radiation; chemical state analysis; biopsy samples; 放射光蛍光X線分析; 毛髪断面; 有機ヒ素; 無機ヒ素; As K-XANES

Synchrotron radiation induced X-ray fluorescence (SR-XRF) analysis was applied to reveal arsenic level in human hair and skin. The samples are obtained from patients of acute and chronic arsenic poisonings. The former specimens were collected from victim of arsenic curry murder occurred at Wakayama city and the latter were from China, where serious arsenic environmental problem exists and chronic poisoning is generated in a few hundred thousands person scale. The distribution of arsenic along the length of a hair was measured by one-dimensional SR-XRF analysis and that in the vertical section was measured by two-dimensional analysis utilizing X-ray microbeam in 5 μm size. The skin samples obtained by biopsy was also examined. Chemical state of arsenic is strongly related with toxicity and therefore it is another important subject of the present study.One dimensional analysis of hairs from victims of arsenic poisoning show that presence of a clear peak of arsenic and high arsenic level … More continued for 1cm length (about two weeks). On the other hand, the results for the samples of chronic poisoning show monotonous distribution of arsenic. Two dimensional analysis of hair section has revealed high accumulation of arsenic at outer rim of the hair whereas zinc and sulfur was uniformly distributed. The distribution of copper was similar to that of arsenic. It is found for the first time that the accumulation of arsenic does happen in the skin from patients of chronic arsenic poisoning. The high arsenic level was observed at a region between corium and epidermis of the skin. XANES analysis of arsenic in the hair of acute poisoning has revealed that arsenic exists in the form of inorganic trivalent state. This result suggests that the arsenic was not metabolized in the acute poisoning. The present study clearly revealed the accumulation mechanism of arsenic in both acute and chronic arsenic poisonings. This study also demonstrated that SR-XRF is a powerful nondestructive technique to reveal distribution and chemical form of trace arsenic in biopsy samples. Less

同步辐射诱导的X射线荧光（SR-XRF）分析用于揭示人头发和皮肤中的砷水平。样品是从急性和慢性砷中毒的患者那里获得的。以前的标本是从砷咖喱谋杀案的受害者那里收集的，后者是从中国那里进行的，后者存在严重的砷环境问题，并以数十万人的规模产生了慢性中毒。通过一维SR-XRF分析来测量砷沿头发长度的分布，并且在垂直部分中，通过利用X射线微束5μm的X射线微束进行测量。还检查了通过活检获得的皮肤样品。砷的化学状态与毒性密切相关，因此它是本研究的另一个重要主题。对侵犯砷中毒的毛发的一个维度分析表明，砷和高砷水平的明显峰值的存在……长度持续了1cm长度（约两周）。另一方面，慢性中毒样品的结果显示砷的单调分布。头发部分的二维分析表明，砷在头发外边缘的高积累，而锌和硫则均匀分布。铜的分布与砷的分布相似。首次发现，慢性砷中毒患者的皮肤确实发生了砷的积累。在皮肤和表皮之间的区域观察到高砷水平。急性中毒头发中砷的Xanes分析表明，砷以无机三价状态的形式存在。该结果表明，砷在急性中毒中没有代谢。本研究清楚地揭示了急性和慢性砷中毒中砷的积累机制。这项研究还表明，SR-XRF是一种强大的非破坏性技术，可揭示活检样品中痕量砷的分布和化学形式。较少的

项目成果

Y.Terada et al.: "Development and application of multi-purpose X-ray Fluorescence analyzer using synchrotron and conventional X-ray sources"X-ray Spectrom.. 28. 461-463 (1999)

Y.Terada 等人：“使用同步加速器和常规 X 射线源的多用途 X 射线荧光分析仪的开发和应用”X 射线光谱.. 28. 461-463 (1999)

Y.Terada: "Development and application of multi-purpose X-ray fluorescence analyzer using synchrotron and conventional X-ray sources"X-ray Spectrom.. 28. 461-463 (1999)

Y.Terada：“使用同步加速器和常规X射线源的多用途X射线荧光分析仪的开发和应用”X射线光谱.. 28. 461-463 (1999)

Y.Terada, et.al.: "Development and application of multi-purpose X-ray Fluorescence analyzer using synchrotron and conventional X-ray sources"X-ray Spectrom. 28. 461-463 (1999)

Y.Terada 等人：“使用同步加速器和常规 X 射线源的多用途 X 射线荧光分析仪的开发和应用”X 射线光谱。