Rapid and Sensitive Microanalyzes of ^<13>C and ^<15>N tracers.

^ 13 C和^ 15 N示踪剂的快速且灵敏的微量分析。

• 批准号: 04554027
• 负责人: WADA Eitaro
• 金额: $ 13.44万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04554027/
• 关键词: Gaschromatograph-Isotope Ratio Mass Spectrometer; Carbon isotope ratio; Nitrogen isotope ratio; Bubble methane; Total inorganic carbon; ^<13>C・^<15>N tracer; Fractionation of microorganisms; Natural abundances carbon and nitrogen isotopes; 炭素・窒素安定

1. Gas chromatography/Combusion/Isotope Ratio Mass Spectrometer (GC/C/IRMS) was successfully established. The system was employed to develop a rapid and simple analytical methods for measuring carbon isotope ratio of bubble methane collected from wetlands, dissolved inorganic carbon (SIGMA CO_2) in lake water samples, and ^<13>C tracer studies of ^<13>C-CH_4 production from a rhizosphere of rice plants.2. ^<15>N_2 and ^<13>C tracer studies were also developed for assessing the molecular dinitrogen fixation by the dry-wod termite, Neotermes koshuenies Shiraki, and the primary production by phytoplankton in Lake Biwa.3. High vacuum line for the prepation of biological meterials were built and carbon and nitrogen isotope ratios were measured for various kinds of biogenic samples collected from L.Biwa, L.Suwa, L.Teganuma and a termite ecosystem.4. Size fractionation of aquatic microorganisms was successfully performed by using a various kind of plankton net and soating by hand.

1。气相色谱/混合/同位素比质谱仪（GC/C/IRMS）成功建立。该系统被用来开发一种快速而简单的分析方法，用于测量从湿地中收集的气泡甲烷的碳同位素比，在湖水样品中溶解的无机碳（Sigma co_2）以及 ^<13> c tracer研究的 ^<13> c-ch_4的tracer研究，从稻米rhizosphere中产生的 ^<13> c-ch_4。 ^<15> n_2和 ^<13> c示踪剂研究还开发了用于评估干wod termite，neotermes koshuenies Shiraki和Biwa湖中浮游植物的主要生产的分子二氮固定。建立了用于生物计量量的高真空线，并针对从L.Biwa，L.Suwa，L.Suwa，L.Teganuma和白蚁生态系统收集的各种生物样品测量了碳和氮同位素比。水生微生物的大小分馏成功通过使用各种浮游生物网和手工进行。

项目成果

和田英太郎: "週刊朝日百科 動物たちの地球 142 地球共生系" 朝日新聞社, 4 (1994)

和田英太郎：《周刊朝日百科全书地球动物142地球共生系统》朝日新闻，4（1994）

Sugimoto A.,E.Wada: "Carbon isotopic composition of bacterial methane in a soil incubation experiment:Contribution of acetate and CO_2/H_2." Geochim.Cosmochim.Acta. 57. 4015-4027 (1993)

Sugimoto A.，E.Wada：“土壤培养实验中细菌甲烷的碳同位素组成：醋酸盐和 CO_2/H_2 的贡献。”

Wada, E., Y.Kabaya and Y.Kurihara: "Stable isotopic structure of aquatic ecosystems." J.Biosci.18. 483-499 (1993)

Wada, E.、Y.Kabaya 和 Y.Kurihara：“水生生态系统的稳定同位素结构。”

Sugimoto A.and E.Wada: "Carbon isotopic composition of bacterial methane in a soil incubation experiment : Contribution of acetate and CO_2/H_2." Geochim.Cosmochim.Acta. 57. 4015-4027 (1993)

Sugimoto A.和 E.Wada：“土壤培养实验中细菌甲烷的碳同位素组成：醋酸盐和 CO_2/H_2 的贡献。”

E.Wada: "Possible impacts of acid rain on aquatic ecosystems from stable isotopic view points." Proceedings of the Int'l Workshop,国立環境研. 117-126 (1993)

E. Wada：“从稳定同位素的角度来看酸雨对水生生态系统的可能影响”，国家环境研究所国际研讨会论文集，117-126（1993）。