Survey of intestinal disaccaridase activities in the wild insectivores and development of disease model for disaccaridase deficiency

野生食虫动物肠道双虫酶活性调查及双虫酶缺乏症模型的建立

基本信息

批准号：
03680038
负责人：
ODA Sen-ichi
金额：
$ 1.22万
依托单位：
Nagoya University, School of Agricultural Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03680038/
关键词：
disaccaridase sucrase insectivore suncus shrew enzyme deficiency disease model 系統育成スクラ-ゼ

项目摘要

1)Intestinal disaccaridase activities were survey in the wild insectivores to develop animal models for human disaccaridase deficiency. Lack of sucrase activities was detected in some insectivore colonies but oher enzyme activities (isomaltase, maltase, and treharase) were present.2)Suncus murinus in Taramajima-island were lack of sucrase activity although all suncus murinus in Sri Lanka, Bangladesh, Okinawa-island and Tokunoshima-island have enzyme activities. We established TR,NAG and MI strains with sucrase deficiency in suncus murimus.3)Lack of sucrase activities in the intestinal brush border membranes was found in Japanese wild Soricinae species such as Sorex caecutiens (1 animal in 1 area), Sorex gracillimus (8 animals in 1 area), Sorex hosonoi (2 animals in 1 area), Sorex sadonis (4 animals in 1 area), Sorex shintto (5 animals in 2 areas), and Sorex unguiculatus (7 animals 1 area). We also found lack of sucrase activities in Chimalogale himalayica (2 animals in 1 area) and Anourosorex squamipes (3 animals in 1 area).4)All disaccaridase activities were detected in Crocidura dsinezumi (4 animals in 2 area), Mogera kobeae (6 animals in 1 area), Mogera wogura (1 animal in 1 areae, Urotrichus talpoides (12 animals in 6 areas) and Dymecodon pilirostris (12 animals in 2 areas).

1)调查野生食虫动物肠道双虫酶活性，建立人类双虫酶缺乏症动物模型。在一些食虫群落中检测到缺乏蔗糖酶活性，但存在其他酶活性（异麦芽糖酶、麦芽糖酶和海藻糖酶）。2）塔拉马岛的 Suncus murinus 缺乏蔗糖酶活性，尽管斯里兰卡、孟加拉国、冲绳的所有 Suncus murinus -岛和德之岛有酶活性。我们在suncus murimus中建立了蔗糖酶缺乏的TR、NAG和MI菌株。3）在日本野生Soricinae物种中发现肠刷状缘膜缺乏蔗糖酶活性，例如Sorex caecutiens（1个地区1只动物）、Sorex gracillimus（8 1 个区域中的动物）、Sorex hosonoi（1 个区域中 2 只动物）、Sorex Sadonis（1 个区域中 4 只动物）、Sorex shintto（2 个区域 5 只动物）和 Sorex unguiculatus（1 个区域 7 只动物）。我们还发现 Chimalogale Himalayica（1 个区域中的 2 只动物）和 Anourosorex squamipes（1 个区域中的 3 只动物）缺乏蔗糖酶活性。4）在 Crocidura dsinezumi（2 个区域中的 4 只动物）、Mogera kobeae（6 只动物）中检测到了所有双糖酶活性。 1 区域中的动物）、Mogera wogura（1 区域中的 1 种动物）、Urotrichus talpoides（12 6 个区域的动物）和 Dymecodon pilirostris（2 个区域的 12 只动物）。