Studies on mechanism of heat production in the house musk shrew (Suncus murinus) with unstable body temperature.

体温不稳定家麝鼩（Suncus murinus）产热机制的研究。

• 批准号: 16500276
• 负责人: ODA Sen-ichi
• 金额: $ 2.24万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500276/
• 关键词: shrew (Suncus murinus); brown adipose tissue; heat production; uncoupling protein-1 (Ucp-1); deiodinase-2 (D2); cold intolerance; 産熱性; 甲状腺ホルモン; UPC-1; 褐色脂肪

The house musk shrew (Suncus murinus), or suncus, is a unique experimental animal that is thought to be cold intolerant. When it is maintained in low temperature room, it becomes hypothermic, immobile, and eventually dies.1) We found that the suncus are more susceptible to cold than mice, and that one reason for their cold intolerance is a malfunction of heat production and lipid depletion in brown adipose tissue (IBAT).2) Neither mild cold exposure nor a high-fat diet alone induced up-regulation of the mRNAs for uncoupling protein 1 (Ucp1) and type II 5'-deiodinase (D2) in BAT. However, combinations of cold exposure and high-fat diet significantly increased both mRNAs. Therefore, cold intolerance in suncus may be partly caused by dietary components.3) We did cDNA cloning of suncus Ucp1 and compared it to other species. The deduced amino acid sequence showed high homology with other mammalian UCP1. Northern blot analysis revealed mRNA in BAT as in other mammals. However, a difference in an amino acid residue was observed in an important residue for thermogenesis. Genomic sequence analysis showed that this difference existed in our two genetically distant laboratory colonies. These results suggest that the cold intolerance in suncus may be derived from low thermogenic activity of UCP1.

麝香鼩（Suncus murinus）或 suncus 是一种独特的实验动物，被认为不耐寒冷。当它被维持在低温房间时，它会变得体温过低，无法活动，最终死亡。 1）我们发现，suncus比小鼠更容易受冷，而它们不耐冷的原因之一是产热和脂质的故障。棕色脂肪组织消耗 (IBAT)。2) 轻度寒冷暴露或单独高脂肪饮食均不会诱导解偶联蛋白 1 (Ucp1) 和 II 型蛋白 mRNA 上调BAT 中的 5'-脱碘酶 (D2)。然而，寒冷暴露和高脂肪饮食的结合显着增加了这两种 mRNA。因此，suncus的冷不耐受可能部分是由饮食成分引起的。3)我们对suncus Ucp1进行了cDNA克隆，并将其与其他物种进行了比较。推导的氨基酸序列与其他哺乳动物UCP1具有高度同源性。 Northern 印迹分析揭示了 BAT 中的 mRNA 与其他哺乳动物中一样。然而，在对生热作用重要的残基中观察到氨基酸残基的差异。基因组序列分析表明，这种差异存在于我们两个基因相距遥远的实验室群体中。这些结果表明，suncus 的冷不耐受可能源于 UCP1 的低产热活性。

项目成果

Change in Ucp1 mRNA expression by long-term cold exposure under normal or high-fat diet feeding in a cold-intolerant mammal,suncus.

在正常或高脂肪饮食喂养下，不耐受寒冷的哺乳动物，suncus 中，由于长期寒冷暴露，Ucp1 mRNA 表达发生变化。

• 发表时间: 2006
• 期刊: Experimental Animals July(in press)
• 作者: Suzuki;D.;Murata Y.;Oda;S
• 通讯作者: S

Change in Ucp1 mRNA expression by long-term cold exposure under normal or high-fat diet feeding in a cold-intolerant mammal, suncus.

在正常或高脂肪饮食喂养下，在不耐冷的哺乳动物桑库斯中长期暴露于寒冷下，Ucp1 mRNA 表达发生变化。

• 发表时间: 2006
• 期刊: Experimental Animals July(in press)
• 作者: Suzuki;D.;Murata Y.;Oda;S.
• 通讯作者: S.