Studies on mechanism of heat production in the house musk shrew (Suncus murinus) with unstable body temperature.

体温不稳定家麝鼩（Suncus murinus）产热机制的研究。

• 批准号: 16500276
• 负责人: ODA Sen-ichi
• 金额: $ 2.24万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500276/
• 关键词: shrew (Suncus murinus); brown adipose tissue; heat production; uncoupling protein-1 (Ucp-1); deiodinase-2 (D2); cold intolerance; 産熱性; 甲状腺ホルモン; UPC-1; 褐色脂肪; shrew (Suncus murinus); brown adipose tissue; heat production; uncoupling protein-1 (Ucp-1); deiodinase-2 (D2); cold intolerance; 産熱性; 甲状腺ホルモン; UPC-1; 褐色脂肪

The house musk shrew (Suncus murinus), or suncus, is a unique experimental animal that is thought to be cold intolerant. When it is maintained in low temperature room, it becomes hypothermic, immobile, and eventually dies.1) We found that the suncus are more susceptible to cold than mice, and that one reason for their cold intolerance is a malfunction of heat production and lipid depletion in brown adipose tissue (IBAT).2) Neither mild cold exposure nor a high-fat diet alone induced up-regulation of the mRNAs for uncoupling protein 1 (Ucp1) and type II 5'-deiodinase (D2) in BAT. However, combinations of cold exposure and high-fat diet significantly increased both mRNAs. Therefore, cold intolerance in suncus may be partly caused by dietary components.3) We did cDNA cloning of suncus Ucp1 and compared it to other species. The deduced amino acid sequence showed high homology with other mammalian UCP1. Northern blot analysis revealed mRNA in BAT as in other mammals. However, a difference in an amino acid residue was observed in an important residue for thermogenesis. Genomic sequence analysis showed that this difference existed in our two genetically distant laboratory colonies. These results suggest that the cold intolerance in suncus may be derived from low thermogenic activity of UCP1.

房子麝香（Musk Shrew）（Muncus murinus）或Suncus是一种独特的实验动物，被认为是冷不宽容的。当它保持在低温室中时，它变得温度不足，不动并且最终死亡。1）我们发现，日烟比小鼠更容易受到寒冷的敏感，而冷不耐受性的原因之一是对棕色脂肪组织中的热量产生和脂质耗尽的失败是棕色脂肪组织（IBAT）的较小饮食（IBAT）。 BAT中的蛋白质1（UCP1）和II型5'-二二醇（D2）。但是，冷暴露和高脂饮食的组合显着增加了两个mRNA。因此，日气中的冷不耐受可能是饮食成分引起的。3）我们进行了suncus ucp1的cDNA克隆，并将其与其他物种进行了比较。推导的氨基酸序列与其他哺乳动物UCP1的同源性很高。 Northern印迹分析表明，与其他哺乳动物一样，蝙蝠中的mRNA。然而，在热生成的重要残基中观察到氨基酸残基的差异。基因组序列分析表明，在我们两个遗传遥远的实验室菌落中存在这种差异。这些结果表明，日气中的冷不耐受可能来自UCP1的低热活性。