Characterizaion and evaluation of soricid mammals : Basic studies on their domestication as unique laboratory animals

群科哺乳动物的特征和评价：作为独特实验动物的驯化基础研究

基本信息

批准号：
06044100
负责人：
ODA Sen-ichi
金额：
$ 3.65万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044100/
关键词：
Shrew Sorex Insectivora Laboratory Animal Russia Genetic Resources

项目摘要

1) We collected 124 insectivores such as Sorex araneus, Sorex caecutiens, Sorex minutus, Sorex isodon, Sorex tundrensis, Neomys fodiens, Asioscalops altaica and Sorex spp.at the field in Novosibirk, Siberia, Russia on July and Aug.1996, and compared with the Japanese shrews.2) The common shrew, Sorex araneus is subdivided into many chromosomal races distinguished by Robertsonian rearrangements. The Novosibirsk race has combination : hn, go, ik, mp. The house musk shrew, Suncus murinus also shows Robertsonian chromosomal variations from chromosomal number 2n=30 to 40. We established the standard karyotype and each Robertsonian fusions of Suncus murinus by the high resolution banding method. Theses two species, Sorex araneus and Suncus murinus are good animal models to estimate the effects of Robertsonian heterozygosity on reproductive performance.3) To study the phylogenic relationships between Sorex species, the nuclear ribosomal DNA spacer and mitochondrial cytochrome b nucleotide seq … More uences were analyzed on Sorex shinto collected in Honshu, Japan and Sorex caecutincecollected in Siberia, Russia. In previous report, these shrews were included in one species, but our results suggested that it is reasonable for the two animals to be divided into distinct species.4) We tried to search for animals with lack of dissacharidase activities. We found that no activities of sucrase were showed in Soricinae shrew such as Sorex araneus, Sorex caecutiens, Sorex minutus, Sorex isodon, Sorex tundrensis, Neomys fodiens collected in Russia and Sorex shinto, Sorex saevus, Sorex sadonis, Sorex unguiculatus collected in Japan, and that presence of sucrase activities were showed in Crocidurinae shrews such as Crocidura sibirica collected in Russia and Crocidura dsinezumi and Suncus murinus except Suncus murinus collected in Nagasaki and Tarama-island, Japan. Lack of sucrase activities in Suncus murinus was controlled by an autosomal single gene. Our results indicates that these animals with no activities of sucrase are potent as an animal model for human disease. Less

1）我们于1996年7月和8月在俄罗斯西伯利亚新西伯利亚野外采集了Sorex araneus、Sorex caecutiens、Sorex minutus、Sorex isodon、Sorex tundrensis、Neomys fodiens、Asioscalops altaica和Sorex spp.等124种食虫动物，并进行了比较与日本鼩鼱。2) 普通鼩鼱，Sorex araneus 被细分为许多染色体种族，以罗伯逊重排为特征。新西伯利亚种族具有 hn、go、ik、mp 组合。家麝鼩、Suncus murinus 也显示出从染色体数 2n=30 到 40 的罗伯逊染色体变异。通过高分辨率显带法对 Suncus murinus 进行标准核型和每个罗伯逊融合。这两个物种，Sorex araneus 和 Suncus murinus 是评估罗伯逊杂合性对繁殖性能影响的良好动物模型。3) 为了研究 Sorex 物种、核核糖体 DNA 间隔区和线粒体细胞色素 b 核苷酸序列之间的系统发育关系……更多影响对在日本本州采集的Sorex shinto和在俄罗斯西伯利亚采集的Sorex caecutince进行了分析，在之前的报告中，这些鼩鼱也被包括在内。 4）我们试图寻找缺乏双糖酶活性的动物，我们发现在鼩鼱等动物中没有显示出蔗糖酶活性。如俄罗斯收集的 Sorex araneus、Sorex caecutiens、Sorex minutus、Sorex isodon、Sorex tundrensis、Neomys fodiens 和 Sorex shinto、Sorex saevus，日本采集的Sorex Sadonis、Sorex unguiculatus，以及在俄罗斯采集的Crocidura sibirica和Crocidura dsinezumi和Suncus murinus等鳄鱼亚科鼩鼱中显示出蔗糖酶活性，但在日本长崎和多良间岛采集的Suncus murinus除外。在 Suncus murinus 中，由常染色体单基因控制，我们的结果表明这些动物没有活动。蔗糖酶作为人类疾病的动物模型是有效的。