Effect of vitamin A on epitheliomesenchymal interaction during odontogenesis and oncogenesis in NMU-treated hamster.

维生素 A 对 NMU 处理的仓鼠牙发生和肿瘤发生过程中上皮间充质相互作用的影响。

• 批准号: 03670842
• 负责人: KOHGO Takao
• 金额: $ 1.28万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670842/
• 关键词: Vitamin A; Disturbances in odontogenesis; Odontogenic tumor; Epitheliomesenchymal interaction; Oncogenesis; N-nitrosomethylurea; Retinol palmitate; beta-carotene; 歯肉腫瘍; 歯の形成異常; ハムスター; 上皮ー間葉相互作用; 上皮ー関葉相互作用; Nーニトロソメチルウレア

1) NMU-induced disturbances in odontogenesis of the cut mandibular incisors was hypoplasia ranging in extent from loss of pigment of enamel and enamel hypoplasia to loss of enamel and irregular hypoplastic dentin formation. These changes of the incisors were more prominent in animals treated with retinol palmitate (RP) plus NMU than those in the animals treated with NMU alone.2) When RP was administered in combination with NMU,there was an increase frequency of odontogenic epithelial cell nests' proliferation with dysplastic dentin formation at the incisal region.3) In RP plus NMU-treated hamsters, the frequency and degree of neoplastic changes of the odontogenic epithelium with dentin formation were increased.4) In NMU-treated hamsters, an excess of beta-carotene slightly prevented the disturbances in odontogenesis, atypical proliferation of odontogenic epithelium, and keratinization of epithelial component.5) NMU inhibited ameloblast differentiation, whereas, the differentiation of odontoblasts and cell-to-cell contact between preameloblasts and odontoblasts were not prevented. RP and beta-carotene did not prevent NMU-induced inhibition of ameloblast differentiation but inhibited NMU-induced keratinization of odontogenic epithelium. Atypical proliferation of odontogenic epithelium with inductive dentin formation caused by RP + NMU treatment resulted from the inhibition of squamous metaplasia with keratinization of the epithelium.6) RP plus NMU treatment resulted to a decrease in incidence of neoplastic invasion of the epithelium in the gingiva or forestomach.7) beta-carotene has moderate suppressive effect on tumor growth of the gingival squamous cell carcinoma and slight effect on squamous cell carcinoma of the forestomach.8) Administration of large quantity of beta-carotene induced adenofibrosislike proliferation of the bile ducts and proliferation of spindle-shaped cells in the submucosa of the glandular stomach in NMU-treated hamsters.

1）NMU诱导的切割下颌切牙牙齿发生的障碍是低浮肿的程度，范围从牙釉质和搪瓷发育不全的色素丧失到牙釉质的丧失和不规则的低肿瘤性牙本质形成。切牙的这些变化在用视黄醇（RP）加上NMU的动物中比单独使用NMU治疗的动物更为突出。2）当RP与NMU结合使用时，肾上腺源性上皮细胞巢的频率增加了3）在RP和NMU治疗的仓鼠中，在切口区域形成了牙本质的增殖。3）在NMU治疗的仓鼠中增加了牙本质源形成的牙源性上皮的频率和程度。 - 耕种略微阻止了牙生物发生的障碍，牙源性上皮的非典型增殖以及上皮成分的角化作用。5）NMU抑制了成木细胞的分化，而牙齿和细胞对细胞对细胞的差异的分化并不限于odemellasts和Odontoblasts之间的细胞接触。 RP和β-胡萝卜素不能阻止NMU诱导的氨基细胞分化抑制，而是抑制了NMU诱导的牙源性上皮的角化作用。由RP + NMU治疗引起的牙本质形成的牙源性上皮的非典型增殖是由鳞状化生的抑制作用而引起的。上皮的角化作用。6）RP Plus NMU治疗导致降低了对上皮或gingiva gingiva gingiva ephiseelium in Gingiva或gingingiva降低的率。 7）β-胡萝卜素对牙龈鳞状细胞癌的肿瘤生长具有中等抑制作用，对Forestomach的鳞状细胞癌的略微影响。8）8）给予大量β-胡萝卜素诱导的诱导的腺染性腺纤维化类似胆管和扩散性的腺触及蛋白。 NMU治疗的仓鼠中腺胃粘膜下张的纺锤形细胞的。