Effect of beta-carotene on epithelio-mesenchymal interaction during ocbntogenesis and oncogenesis in NMU-treated hamster

β-胡萝卜素对 NMU 处理的仓鼠在发病和肿瘤发生过程中上皮间质相互作用的影响

基本信息

批准号：
06671800
负责人：
KOHGO Takao
金额：
$ 1.34万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

We have reported the induction of disturbances in odontogenesis and odontogenic tumors in incisors of NMU-treated hamsters, and also demonstrated the effects of vitamin A on tumorigenesis and disturbances in odontogenesis. In the present study, we investigated the effect of natural and synthetic beta-carotene on NMU-treated hamsters. Six hours after administration of beta-carotenes, NMU treatment was conducted twice a week for three months. The NMU-induced disturbances in the odontogenesis of mandibular incisors were characterized by hypoplastic changes ranging from enamel hypoplasia to loss of enamel and irregular hypoplastic dentin formation. In NMU-treated hamsters, excess administration of beta-carotene slightly repressed disturbances in odontogenesis, atypical proliferation of odontogenic epithelium, and keratinization of epithelial components. In NMU inhibited ameloblast differentiation, the differentiation on odontoblasts and cell to cell contact between preameloblasts and odontoblasts were not prevented. The beta-carotene did not prevent NMU-induced inhibition of ameloblast differentiation but inhibited NMU-induced keratinization of odontogenic epithelium. The beta-carotene had a moderate suppressive effect on the development of the odontogenic tumors. Synthetic beta-carotene seems to be slightly more effective on NMU-induced disturbances in odontogenesis than natural beta-carotene. The beta-carotene may play a role in the normal differentiation of odontogenic epithelial tissue as a biological function and as a provitamin A function. This study may suggest inhibitory effects of beta-carotene on odontogenic tumor development as a biological function rather than as a provitamin A function.

我们报道了 NMU 处理的仓鼠门牙中诱导牙发生紊乱和牙源性肿瘤的情况，并且还证明了维生素 A 对肿瘤发生和牙发生紊乱的影响。在本研究中，我们研究了天然和合成 β-胡萝卜素对 NMU 处理的仓鼠的影响。施用 β-胡萝卜素 6 小时后，每周进行两次 NMU 治疗，持续三个月。 NMU 引起的下颌切牙牙本质发育障碍的特征是发育不全的变化，范围从牙釉质发育不全到牙釉质丧失和不规则发育不全的牙本质形成。在 NMU 处理的仓鼠中，过量施用 β-胡萝卜素会轻微抑制牙生成的紊乱、牙源上皮的非典型增殖和上皮成分的角化。在NMU抑制成釉细胞分化中，成牙本质细胞的分化以及前成釉细胞和成牙本质细胞之间的细胞间接触没有被阻止。 β-胡萝卜素不能阻止NMU诱导的成釉细胞分化抑制，但抑制NMU诱导的牙源性上皮角化。 β-胡萝卜素对牙源性肿瘤的发展具有中等抑制作用。合成 β-胡萝卜素似乎比天然 β-胡萝卜素对 NMU 引起的牙发育紊乱更有效。 β-胡萝卜素可能作为生物学功能和维生素原A功能在牙源性上皮组织的正常分化中发挥作用。这项研究可能表明 β-胡萝卜素对牙源性肿瘤发展的抑制作用是一种生物学功能，而不是维生素原 A 功能。