Estrogen receptor expression and endocrine disruptors correlates with behavior and histological features of malignant epithelial tumors of the salivary gland

雌激素受体表达和内分泌干扰物与唾液腺恶性上皮肿瘤的行为和组织学特征相关

基本信息

批准号：
12470395
负责人：
KOHGO Takao
金额：
$ 6.53万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

1) Apoptosis and proliferation of parenchymal cells during atrophy of rat submandibular gland induced by double duct ligation were investigated. At 2 and 3 days after ligation, increased PCNA positive cells and mitoses were seen in ducts; thereafter PCNA positive cells decreased in number. At After 7 days, most acinar cells had disappeared; a few acinar cells remained, especially at the lobule periphery. Marked depletion of acinar cell by apoptosis and a concurrent short-lived cycle of duct cell proliferation were seen. Both apoptosis and proliferation of myoepithelial cells occurred, especially in the early phase of atrophy.2) We investigated a sex differences and the effects of ovariectomy on the histology of 9,10-dimethyl-1,2-benzanthracene (DMBA)-induced adenocarcinomas in the submandibular gland of rats. Wistar rats were divided four groups : (1) non ovariectomy, (2) ovariectomy(OV), (3) anti-estrogen Tamoxifen application after development of DMBA-induced adenocarcinoma (4) male … More rats. In Ov rats, induction of adenocarcinoma was significantly reduced but the incidence of squamous cell carcinomas significantly increased. Moreover, the results of anti-estrogen tamoxifen application showed similar changes as those seen in Ov rats. As a ovarian function, estrogen seemed to play an important role in the tumorigenesis and transformation of adenocarcinoma in female rat.3) Clinicopathologically, mucoepidermoid carcinomas were investigated. The sections were immunostained with ER, progesterone receptor (PgR), MIB-1, and cytokeratin (CK) 6,7,8,13, and 19 antibodies. The rate of ER positivity was larger in epidermoid cells than in intermediate cells. The percentage of ER positivity was higher in the low-grade tumors than in the high-grade ones. The MIB-1 positivity was significantly higher in high-grade than in low-grade tumors. ER expression was associated with lymph node metastasis in male mucoepidermoid carcinomas.The expression of different types of CKs and ER suggests that ER is related to cellular differentiation in mucoepidermoid carcinomas. Less

1）凋亡和实质细胞在连接后三天诱导的大鼠下颌腺萎缩，在7天后看到PCNA阳性细胞和有丝分裂的增加；并出现了与萎缩的早期萎缩的导管细胞增殖的同时循环。2）我们研究了性别差异和卵巢对组织学的影响大鼠的下颌腺中的腺癌分为四组：（1）非卵巢症（2）卵巢（2）Ovariente（OV），（3）DMBA诱导的腺癌（4）男性。腺癌的腺瘤显着降低，但叶氧蛋白瘤的癌症明显增加，孕酮受体（PGR），MIB-1和细胞角蛋白（CK）6,7,8,13和19抗表皮细胞中的阳性率。高级别的MIB-1阳性与低级别的肿瘤相比，在雄性粘膜癌中具有淋巴结转移。较少的