Histopathological evaluation of the effects of sex hormones in DMBA-induced rat malignant salivary gland tumors with morphological transformation.

性激素对 DMBA 诱导的大鼠恶性唾液腺肿瘤形态转化影响的组织病理学评价。

基本信息

批准号：
14370600
负责人：
KOHGO Takao
金额：
$ 6.34万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

This study examines whether the sex steroid hormone can cause histopathological changes in experimental DMBA-induced rat carcinomas, using histopathological and immunohistochemical techniques. Wistar rats were divided into 6 groups ; A1) an after orchidectomy adenocarcinoma induction group, A2) a group with prescribed diethylstilbestrol (DES), B) an after adenocarcinoma induction orchidectomy group, C1) an after ovariectomy(OV) adenocarcinoma induction group, C2) a group with prescribed DES, D) an after adenocarcinoma induction OV group.In Ov rats, induction of adenocarcinoma was significantly reduced but the incidence of squamous cell carcinomas significantly increased. Ov rats showed an arrest of growth of adenocarcinoma and a temporary regression of the tumor, consequently, high ratio of squamous cell carcinoma was seen. The results confirm that DMBA-induced adenocarcinoma in the submandibular glands of rats seemed to be sex dependent.The rate of ER positivity was larger in adenocar … More cinomatous lesions than in squamous cell carcinoma. The percentage of ER positivity was higher in the adenocarcinomatous lesions than in the squamous cell carcinomas. The expression of ER is related to cellular differentiation in mucoepidermoid-like carcinomas.The orchidectomy, OV, and DES injected samples showed various cellular and histological characteristics. There were differences in the expressions of CKs, PCNA, and S-100 protein in a similar histological changes. It is suggested that ER plays a significant role in various morphological transformations of malignant salivary gland tumors.Background : The proliferation and distribution of myoepithelial cells and the roles of apoptosis and mitosis of acinar and duct cells in the atrophy of the sublingual gland of rat induced by double duct ligation were investigated using immunohistochemistry. The transition from acinar to duct cell and the necrosis of acinar cells play important roles in the atrophy of the sublingual gland. The distribution of myoepithelial cells change during atrophy of rat sublingual glands. Less

本研究使用组织病理学和免疫组织化学技术检查性类固醇激素是否会引起实验性 DMBA 诱导的大鼠癌症的组织病理学变化，将 Wistar 大鼠分为 6 组；A1）睾丸切除术后腺癌诱导组，A2）服用己烯雌酚的组。（DES），B）腺癌诱导后睾丸切除组，C1）卵巢切除（OV）腺癌诱导后组，C2）使用处方DES的组，D）腺癌诱导后的OV组。在Ov大鼠中，腺癌的诱导显着减少，但鳞状细胞癌的发生率显着增加。肿瘤暂时消退，因此鳞状细胞癌的比例较高，结果证实DMBA诱导的大鼠颌下腺腺癌似乎是性别性的。腺癌中 ER 阳性率高于鳞状细胞癌腺癌中 ER 阳性率高于鳞状细胞癌 ER 的表达与粘液表皮样细胞的分化有关。睾丸切除、OV和DES注射样本显示出不同的细胞和组织学特征，CKs、OV和DES的表达存在差异。 PCNA和S-100蛋白的组织学改变相似，提示ER在唾液腺恶性肿瘤的各种形态学转变中发挥着重要作用。背景：肌上皮细胞的增殖和分布以及腺泡细胞凋亡和有丝分裂的作用。采用免疫组织化学方法研究了双导管结扎引起的大鼠舌下腺萎缩中导管细胞从腺泡到导管细胞的转变。腺泡细胞坏死在舌下腺萎缩中起重要作用。大鼠舌下腺萎缩时肌上皮细胞分布发生变化。