Ultrastructure and kinetics of the apical vesicles of the stria vascularis epithelial cell of the inner ear.

内耳血管纹上皮细胞顶泡的超微结构和动力学。

• 批准号: 03670012
• 负责人: OKADA Teruhiko
• 金额: $ 1.41万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670012/
• 关键词: Stria vascularis; Cationized ferritin; Histochemistry; ACPase; TMPase; Na-K-ATPase; Ca-ATPase; Mg-ATPase; Mg-ATPase; CaーATPase; MgーATPase; NaーKーATPase

The localization of ion-transporting enzyme activity and the endocytic process of cationized ferritin (CF) were electron microscopically investigated in the guinea pig stria vascularis.Na-K-ATPase activity was localized in basolateral infolding of marginal cells of the sria vascularis. Ca-ATPase activity was detected in the limiting membrane of the large endosomal vacuoles and the luminal plasma membrane of the marginal cells. Mg-ATPase activity was found in the lateral plasma membrane of the marginal cells.CF was first observed scattered on the luminal surface of the marginal cells. Then it was internalized in the form of coated vesicles and transported to the large vacuolar endosomes. At 5 min after CF injection, CF particles were mainly localized in the coated vesicles, and at 15 min, they appeared in large vacuoles. At 60 min, multivesicular bodies provided with several internal vesicles were havily labeled with CF.At 2 hours, the content of the multivesicular bodies condensed and internal vesicles, a granular substance of moderate to high density and membrane remants become clearly visible. These multivesicular bodies were havily labeled with clustered CF particles.

在豚鼠血管中，通过电子显微镜研究了电子显微镜（CF）的定位和阳离子铁蛋白（CF）的内吞作用过程。NA-K-ATPase活性定位于Sria Vascularis边缘细胞的基底外侧插入中。在大型内体液泡的限制膜和边缘细胞的腔质膜中检测到CA-ATPase活性。在边缘细胞的外侧质膜中发现了Mg-ATPase活性。CF首先观察到散射在边缘细胞的腔表面上。然后以涂层囊泡的形式将其内化，并运输到大液泡内体。注射CF后5分钟，CF颗粒主要定位在涂层囊泡中，在15分钟时，它们出现在大型液泡中。在60分钟时，提供了几种内部囊泡的多囊体，用CF贴上了CF的标记。在2小时，多尖体的含量和内部囊泡，一种中度到高密度和膜隔音的颗粒状物质变得清晰可见。这些多囊体被簇的CF颗粒标记。

项目成果

T.Okada et al.: "Endocytosis of cationized ferritin in the marginal cells of the guinea pig stria vascularis." Journal of Electron Microscopy. 41. 320- (1992)

T.Okada 等人：“豚鼠血管纹边缘细胞中阳离子铁蛋白的内吞作用。”

T.Okada, T.Kobayashi, E.Garcia and H.Seguchi: "Endocytosis of cationized ferritin in the marginal cells of the guinea pig stria vascularis" Journal of Electron Microscopy. 41(4). 320 (1992)

T.Okada、T.Kobayashi、E.Garcia 和 H.Seguchi：“豚鼠血管纹边缘细胞中阳离子铁蛋白的内吞作用”电子显微镜杂志。

N.Kanoh et al.: "Ultracytochemical study of ouabain-sensitive K^+-dependent p-nitrophenylphosphatase activity in stria vascularis of reserpinized guinea pig." Acta Oto-laryngologica (Stockholm). 113. (1993)

N.Kanoh 等人：“利血平豚鼠血管纹中哇巴因敏感 K+依赖性对硝基苯磷酸酶活性的超细胞化学研究”。