Lectin binding sites in the stria vascularis of normal and endolymphatic hydrops-bearing guinea pigs.

正常和内淋巴积水豚鼠血管纹中的凝集素结合位点。

基本信息

批准号：
01480408
负责人：
SEGUCHI Harumichi
金额：
$ 4.48万
依托单位：
Kochi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

In order to clarify the function of the marginal cells in the guinea pig stria vascularis, the following three experiments were performed :1. Lectins were employed to determine the localization of specific carbohydorates on thin sections of Lowicryl K4M embedded materials. SBA, HPA and RCA-II labeling (Gal-NAc and Gal) was observed in many apical vesicles, endosomes and apical tubules as well as on the microvilli and micropinocytotic invaginations of the luminal plasma membrane of the marginal cells. LFA labeling (sialic acid) was found on the plasma membrane of the basal infoldings of the marginal cells. WGA (Glu-NAc) was detected along the plasma membrane of all types of cells constituting the stria vascularis. The fact that the membranes of internalization, many of the apical vesicles, endosomes and apical tubules in the marginal cells possess the same carbohydrate moieties suggested that these structures. may be associated together.2. The uptake of cationized ferritin (CF) by the a … More pical vesicles in the marginal cells of the stria vascularis was electron microscopically studied. CF particles were found in clusters, scattered on the free surface plasma membrane facing the endolymph and in the caveolae of the marginal cells. They were also detected in the apical vesicles, coated vesicles and endosomes. These findings suggested that the apical vesicles are associated with the free surface plasma membrane, and that they are related to the efficient exchange of ions between the endolymph and the marginal cells.3. The effect of endolymphatic hydrops on the Na-K-ATPase activity in the stria vascularis was cytochemically investigated one year after experimental induction. The morphological observations revealed intercellular dropsy and shrinkage of the basal infoldings of the marginal cells, and shrinkage and disappearance of intermediate cells. In spite of such morphological alterations, no remarkable difference was found, as regards the localization of the Na-K-ATPase activity, between the striae vasculares of hydrops and control specimens. Less

为了澄清豚鼠血管中的边缘细胞，进行了以下三个实验：1。确定在lowicryl k4m嵌入材料的特定碳水化合物中的定位。 NAC和GAL在许多顶部的囊泡中观察到了边缘细胞的腔膜。边缘细胞相同的碳水化合物部分提出了结构。2。表面质膜在边缘细胞的内膜和caveolae中被检测到的LSO膜，覆盖的囊泡和内体。内淋巴和边缘细胞之间的离子。3。边缘和snd hrinkage和中间细胞的ization ization