Multipatch Setup

多面体设置

基本信息

批准号：
524345021
负责人：
金额：
--
依托单位：
Johannes Gutenberg-Universität Mainz
依托单位国家：
德国
项目类别：
Major Research Instrumentation
财政年份：
2023
资助国家：
德国
起止时间：
2022-12-31 至无数据
项目状态：
未结题

来源：
https://gepris.dfg.de/gepris/projekt/524345021?language=en
关键词：
Multipatch Setup

项目摘要

We hereby apply for the procurement of a multipatch setup that will enable neuroscience researching groups at the University Medical Center Mainz to carry out complex electro-physiological experiments. A multipatch setup enables a direct analysis of network parameters such as connectivity, excitation and inhibition, time-precise detection of individual action potentials, and the analysis of synapse properties (short-term plasticity, synaptic strength, etc.) in acute brain slices. In principle, the analysis of synaptic communication is also possible on conventional setups with recording form 2 neurons. However, these measurements are very time-consuming, since the probability of finding monosynaptically connected pairs in acute brain slices is extremely low. With the proposed multipatch setup, up to 12 neurons can be recorded simultaneously. In addition, the pipettes on the setup can be cleaned automatically after each recording such that one can record with one pipette signals from several neurons in succession. This drastically increases the number of neuron connections that can be tested in one experiment (>100). With the help of a multipatch setup, large amounts of electrophysiological data can be obtained in a short time with a significantly reduced number of test animals. This will enable us to carry out a comprehensive analysis of complex network parameters in acute brain slices, which would not be possible with conventional setups the high expenditure of time.

我们特此申请采购多补丁装置，使美因茨大学医学中心的神经科学研究小组能够进行复杂的电生理实验。多面体设置可以直接分析网络参数，例如连接性、兴奋和抑制、个体动作电位的时间精确检测以及急性脑切片中突触特性（短期可塑性、突触强度等）的分析。原则上，在记录 2 型神经元的传统设置上也可以对突触通讯进行分析。然而，这些测量非常耗时，因为在急性脑切片中找到单突触连接对的概率极低。通过所提出的多面体设置，最多可以同时记录 12 个神经元。此外，装置上的移液器可以在每次记录后自动清洁，这样就可以用一根移液器连续记录来自多个神经元的信号。这极大地增加了一次实验中可测试的神经元连接数量（>100）。借助多补丁设置，可以在短时间内获得大量电生理数据，同时显着减少测试动物的数量。这将使我们能够对急性脑切片中的复杂网络参数进行全面分析，这在传统设置中是不可能实现的，而且需要花费大量时间。