LC/MS2 Setup for Standard, as well as High Sensitivity Proteomics Work-Flows

适用于标准和高灵敏度蛋白质组学工作流程的 LC/MS2 设置

• 批准号: 533391421
• 金额: --
• 依托单位: Philipps-Universität Marburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/533391421?language=en
• 关键词: LC; MS2; Setup; Standard; well

The global analysis of protein mixtures using mass spectrometry-based methods hasevolved into a corner stone of biomedical research. At the Department of Medicine ofthe Philipps-Universität of Marburg, such analyses are intensively used and performedin cooperation with the Service Group Mass Spectrometry and Elemental Analysis atthe Department of Chemistry. The current sample spectrum ranges in complexity andcorrelated analytical demand from affinity purified protein complexes, via whole cell andtissue lysates to global analyses of post-translational modifications (PTMs).In the context of the new establishment of an Institute and Core Facility of TranslationalProteomics at the Department of Medicine (since 10.2021), a high resolution massspectrometer setup is targeted including liquid chromatography. The instruments are in-tended to a) expand existing instrument capacity, which is currently insufficient to meethigh and growing demand and b) extend capabilities towards tackling analytes of ex-tremely low availability, in particular single cell proteomics approaches.

使用基于质谱的方法对蛋白质混合的全球分析已融合到生物医学研究的角石。在马尔堡的菲利普斯 - 纳弗里蒂特医学系，此类分析被深入使用，并与化学系的服务组质谱和元素分析进行​​了合作。当前的样品光谱在复杂性和相关性需求之间从亲和纯化的蛋白质复合物中，通过全细胞和组织裂解物到全球翻译后修饰（PTMS）的全球分析（PTMS）。在新建立的机构和核心核心设施的新建立的背景下，医学部门的液体统计学范围（自10.202111）的核心群体设置了较高的群体，该设施群群落群群落群群落。这些仪器倾向于a）扩大现有的仪器能力，目前不足以使Meethig和需求不断增长，b）扩展了能力，以应对处理前较低可用性的分析物，尤其是单细胞蛋白质组学方法。