Factors of Interaction between Osteoblasts and Bone Marrow Cells in Bone Remodeling

成骨细胞与骨髓细胞在骨重塑中相互作用的因素

• 批准号: 01571113
• 负责人: MITANI Hideo
• 金额: $ 1.34万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571113/
• 关键词: Osteoblasts; Bone marrow Cells; Bone Remodeling; Co-Culture; Facs; ALPase Activity; MAC-1; LFA-1; coーculture; LFAー1抗原; 生理的骨改造機構; co-culture; Mac-1; LFA-1; ALPase活性

The purpose of this investigation was to reveal the physiological bone remodeling mechanism by means of cellular interaction phenomenon between osteoblasts (OB) and bone marrow cells (BM) in vitro.When OB ( 1X10^6 cells) obtained from 4 weeks old mouse long bones were co-cultivated by using millicell as separator in CO_2 incubator for 14 days, the number of BM was increased and the high incorporation of ^3H-thymidine into cellular DNA of BM was markedly recognized after 3 day incubation. However, the number of BM and uptake of ^3H-thymidine into BM did not increase in BM only cultivation. The increased BM were identified to have surface antigens of Mac-1 and LFA-1 by FACS analysis. But, ALPase activity of OBco-cultivated with BM was remarkably suppressed after 3 days incubation and reduced to one half of that of control after 5 days incubation. These results clearly indicated that both OB and BM produced some differential factors of the clonal growth of Mac-1 and LFA-1 positive cells from BM cells and suppression of ALPase of OB in co-cultivation with OB and BM. It was suggested that the factors produced from interaction between OB and BM might regulate the mechanism of physiological bone remodeling.

本研究的目的是通过体外成骨细胞（OB）和骨髓细胞（BM）之间的细胞相互作用现象来揭示生理性骨重塑机制。当从4周龄小鼠长骨中获得OB（1X10^6细胞）时以millicell为隔膜在CO_2培养箱中共培养14天，BM数量增加，且^3H-胸苷高度掺入BM细胞DNA中孵育3天后明显识别。然而，在仅BM培养中，BM的数量和BM摄取^3H-胸苷并没有增加。通过FACS分析鉴定增加的BM具有Mac-1和LFA-1的表面抗原。但是，与BM共培养的OB的ALPase活性在孵育3天后被显着抑制，并且在孵育5天后降至对照的一半。这些结果清楚地表明，OB和BM都产生了来自BM细胞的Mac-1和LFA-1阳性细胞的克隆生长的一些差异因子，以及在与OB和BM共培养时抑制OB的ALPase。提示OB和BM相互作用产生的因子可能调节生理性骨重塑机制。

项目成果

千葉 美麗,清水 義之,三谷 英夫: "Mechanical stressによる骨改造機構における骨芽細胞と骨髄細胞の相互作用の解析" 日本矯正歯科学会雑誌. 48. 677 (1989)

Mirei Chiba、Yoshiyuki Shimizu、Hideo Mitani：“机械应力诱导的骨重塑机制中成骨细胞和骨髓细胞之间的相互作用分析”日本正畸学会杂志 48. 677 (1989)。

千葉美麗: "Mechanical stressによる骨改造機構の解明1.Compressive forceを負荷した新生ラット培養長管骨における破骨細胞の分化誘導について" 日本骨代謝学会雑誌. 7S. 211 (1989)

Mirei Chiba：“机械应力诱导的骨重塑机制的阐明1.在受压力的新生大鼠培养的长骨中诱导破骨细胞分化”日本骨代谢学会杂志211（1989）。

Chiba,M.,Shimizu,Y.,Shimizu,Y.and Mitani,H.: "A study of bone remodeling mechanism induced by mechanical stress IーDifferentiation osteoclasts induced by copmressive forcein new bone rat cultured long boneー" J Bone and Mineral Metabolism. 8. 48 (1990)

Chiba, M., Shimizu, Y., Shimizu, Y. and Mitani, H.：“机械应力诱导的骨重塑机制的研究I－压缩力诱导新生骨大鼠培养长骨中的分化破骨细胞－” J Bone and矿物质代谢。8. 48 (1990)

Chiba, M., Shimizu, Y., Simizu, Y., Mitani, H.: "A study of bone remodeling mechanism induced by mechanical stress. 1. Differentiation of osteoclasts induced by compressive force in newborn rat cultured long bone" J Bone Mine Metab. 8. 48 (1990)

Chiba, M.、Shimizu, Y.、Simizu, Y.、Mitani, H.：“机械应力诱导的骨重塑机制的研究。1.新生大鼠培养长骨中压缩力诱导的破骨细胞的分化”J Bone

清水義之: "Mechanical stressによる骨改造機構の解明II.骨改造機構における骨芽細胞と骨芽細胞の相互作用因子の解析" 日本骨代謝学会雑誌. 7S. 207 (1989)

Yoshiyuki Shimizu：“机械应力诱导的骨重塑机制的阐明II。骨重塑机制中成骨细胞和成骨细胞之间的相互作用因素的分析”日本骨代谢学会杂志207（1989）。