规则有序碳纳米管3D结构及其内构电场对干细胞成骨分化的影响

The hierarchical and well-aligned structure is of great significance for the realization of biological tissue function and the maintenance of mechanical properties. In the bone tissue, there are more widely distributed nano- and micro- porous structures. In the past, from the perspective of physical signals, the effects of structure on stem cell differentiation were mostly based on two-dimensional (2D) design and regulation. Therefore, It is unable to explore and regulate the growth and differentiation of cells or tissues in 3D space. This project designs and constructs a hierarchical and well-aligned 3D structure: the focus is on oriented carbon nanotubes. Through parameter control and micro-nano processing technology, the vertically oriented nanostructures are firstly produced, with adjustable pore size and shape in micrometer scales. By assembling the extension electrode and utilizing the cellular electrophysiological characteristics, the stem cells are micro-electrically regulated in a stereoscopic space to achieve a 3D ordered osteogenic induction. This project implementation will clarify the influence and regulation of physical hierarchical and well-aligned structure and micro-electric environment on stem cell bone differentiation in 3D micro-nano space, and provide theoretical and experimental basis for repairing bone tissue regeneration from the perspective of micro-nano structure bionics.

多级规则有序结构对生物组织功能实现、力学性能保持具有重要意义。骨组织中更是广泛存在着取向排列的纳米和微米多孔结构。以往从物理信号角度出发，研究结构对干细胞骨向分化的影响，大多基于二维空间（2D）设计和调控。无法探寻和调节细胞或组织在3D空间的生长和分化。本项目设计构建一种多级规则有序3D结构：重点以取向碳纳米管为基础，通过参数调控和微纳加工技术，首先实现垂直取向的纳米结构，并在微米尺度上实现可调孔径大小和形状。通过组装扩展电极，利用干细胞电生理特征，在立体空间中对干细胞进行微电调控，实现3D有序规则成骨诱导作用。项目实施将明确在3D微纳米空间中物理规则有序结构和微电环境对干细胞骨向分化的影响和调控，为未来从微纳米结构仿生学角度修复骨组织再生提供理论和实验依据。

多级规则有序结构对生物组织功能实现、力学性能保持具有重要意义。骨组织中更是广泛存在着取向排列的纳米和微米多孔结构。以往从物理信号角度出发，研究结构对干细胞骨向分化的影响，大多基于二维空间（2D）设计和调控。无法探寻和调节细胞或组织在3D空间的生长和分化。本项目设计构建一种多级规则有序3D结构：重点以取向碳纳米管为基础，通过参数调控和微纳加工技术，首先实现石墨烯负载石墨烯碳管结合的微纳结构，并在微米尺度上仿生骨小梁空隙微米空间。项目实施明确了3D石墨烯负载碳纳米管（3DGp/CNTs）微纳米空间促进牙髓干细胞（DPSCs）骨向分化的正向调控作用，同时探究了其通过上调BMPs信号通路，级联调控DPSCs的成骨分化。为未来从微纳米结构仿生学角度修复骨组织再生提供理论和实验依据。

内容获取失败，请点击重试