Regulatory mechanism of MMPs expression in periodontal ligament cells induced by mechanical stress

机械应力诱导牙周膜细胞MMPs表达的调控机制

基本信息

批准号：
12671986
负责人：
MITANI Hideo
金额：
$ 2.5万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671986/
关键词：
MMPs (Matrix Metalloproteinases)Mechanical stress Periodontal tissue Remodeling Periodontal ligament cells RT-PCR in situ hybridization Orthodontic tooth movement Gene expression MMOs(Matrix Metalloproteinases)骨芽細胞細胞外基質 in situ hy

项目摘要

The purpose of this investigation is to clarify which MMPs are involved in the periodontal tissue remodeling during tooth movement and how the expression and activation of MMPs are regulated. Especially, direct induction of gene expression of any MMPs will be focused on, not only verify the indirect pathway through cytokine signaling.1. We employed an animal model system to elucidate the histological changes during the tooth movement and changes in the gene expression patterns of MMPs by using in situ hybridization method. We found that the expression of MMPs-2, -8 and -13 were transiently promoted in the PDL cells, while the MMP-9 is only expressed in the osteoclasts.2. Human PDL cells were subjected to two different type of mechanical stress ; tension-force and compressive-force, and showed similar pattern of mRNA expression. MMP-1 and -2 were up-regulated. Membrane type MMP, MT1-MMP was also detectable, and its expression level was not changed by mechanical stress. MMP-9 was not detected in this experiment. Only TIMP-1 mRNA Level was stable after mechanical stress. On the other hand, mRNA expression levels of TIMP-1, -2 were up-regulated. The changes of mRNA expression level showed similar pattern with the changes of mRNA level by the treatment of prostaglandin E2. Inducible prostaglandin synthase, COX-2 mRNA expression was also up-regulated by mechanical stress.

这项研究的目的是澄清牙齿运动过程中哪些MMP参与牙周组织重塑，以及如何调节MMP的表达和激活。特别是，任何MMP的基因表达的直接诱导都将集中在侧重于通过细胞因子信号传导验证间接途径1。我们采用了动物模型系统来阐明牙齿运动过程中的组织学变化，并使用原位杂交方法使用了MMP的基因表达模式。我们发现MMPS -2，-8和-13的表达在PDL细胞中瞬时促进，而MMP -9仅在破骨细胞中表达。2。人类PDL细胞受到两种不同类型的机械应力。张力和压缩力，并显示出相似的mRNA表达模式。 MMP-1和-2上调。还可以检测到膜型MMP，MT1-MMP，其表达水平不会因机械应力而改变。在此实验中未检测到MMP-9。在机械应力后，只有TIMP-1 mRNA水平稳定。另一方面，TIMP-1，-2的mRNA表达水平被上调。 mRNA表达水平的变化通过治疗前列腺素E2的治疗显示与mRNA水平的变化相似。机械应力也将诱导前列腺素合酶，Cox-2 mRNA表达上调。