Regulatory mechanism of MMPs expression in periodontal ligament cells induced by mechanical stress

机械应力诱导牙周膜细胞MMPs表达的调控机制

基本信息

批准号：
12671986
负责人：
MITANI Hideo
金额：
$ 2.5万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671986/
关键词：
MMPs (Matrix Metalloproteinases)Mechanical stress Periodontal tissue Remodeling Periodontal ligament cells RT-PCR in situ hybridization Orthodontic tooth movement Gene expression MMOs(Matrix Metalloproteinases)骨芽細胞細胞外基質 in situ hy

项目摘要

The purpose of this investigation is to clarify which MMPs are involved in the periodontal tissue remodeling during tooth movement and how the expression and activation of MMPs are regulated. Especially, direct induction of gene expression of any MMPs will be focused on, not only verify the indirect pathway through cytokine signaling.1. We employed an animal model system to elucidate the histological changes during the tooth movement and changes in the gene expression patterns of MMPs by using in situ hybridization method. We found that the expression of MMPs-2, -8 and -13 were transiently promoted in the PDL cells, while the MMP-9 is only expressed in the osteoclasts.2. Human PDL cells were subjected to two different type of mechanical stress ; tension-force and compressive-force, and showed similar pattern of mRNA expression. MMP-1 and -2 were up-regulated. Membrane type MMP, MT1-MMP was also detectable, and its expression level was not changed by mechanical stress. MMP-9 was not detected in this experiment. Only TIMP-1 mRNA Level was stable after mechanical stress. On the other hand, mRNA expression levels of TIMP-1, -2 were up-regulated. The changes of mRNA expression level showed similar pattern with the changes of mRNA level by the treatment of prostaglandin E2. Inducible prostaglandin synthase, COX-2 mRNA expression was also up-regulated by mechanical stress.

本研究的目的是阐明哪些 MMP 参与牙齿移动过程中的牙周组织重塑，以及 MMP 的表达和激活是如何调节的。特别是，直接诱导任何MMPs的基因表达将受到关注，而不仅仅是验证通过细胞因子信号传导的间接途径。 1．我们采用动物模型系统，通过原位杂交方法阐明牙齿移动过程中的组织学变化以及MMPs基因表达模式的变化。我们发现PDL细胞中MMPs-2、-8和-13的表达短暂升高，而MMP-9仅在破骨细胞中表达。2．人类 PDL 细胞受到两种不同类型的机械应力；张力和压力，并显示出相似的 mRNA 表达模式。 MMP-1 和-2 上调。膜型MMP、MT1-MMP也可检测到，且其表达水平不因机械应力而改变。本实验未检测到MMP-9。只有 TIMP-1 mRNA 水平在机械应力后保持稳定。另一方面，TIMP-1、-2的mRNA表达水平上调。 mRNA表达水平的变化与前列腺素E2处理后mRNA表达水平的变化具有相似的规律。诱导性前列腺素合酶、COX-2 mRNA 表达也因机械应激而上调。