Immunohistochemical Study of Age-dependent Changes in the Gingival Tissue

牙龈组织年龄依赖性变化的免疫组织化学研究

基本信息

批准号：
01571069
负责人：
SAKAI Takako
金额：
$ 1.34万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571069/
关键词：
Aging change Periodontal tissue Type I and type III collagens Fibronectin コラ-ゲン加齢

项目摘要

Immunofluorescent and immunoelectron microscopic studies of age-dependent changes of the periodontal tissues were performed in the lower first molars of mice ranging in age from one week to eighty weeks after birth. The alveolar bone which was completed by 20 weeks, was gradually decreased in amount and its surface bordered upon the periodontal ligament became irregular resulting in wavy appearance. A space which was occupied by the periodontal ligament decreased in width with aging, probably because of deposition of newly formed cementum on the surface of the root. In addition, cells in the periodontal ligament and in the gingival tissue also decreased in number with aging.The typeI and type Ill collagens, and fibronectin were distributed in close association with the fibers of the periodontal tissue and the gingival tissue. TypeIIl collagen was intensely stained in association with the fiber bundles of the periodontal ligament being close to the alveolar bone of mice ranging in age from 2 to 20 weeks after birth. This fact indicates that an active syntheses of type III collagen occurred in this region. In mice of after 30 weeks postpartum, the junctional epithelium proliferated and migrated down to the apical side along the cementum. The fibronectin was strongly stained on the external basement membrane of migrating junctional epithelium. This finding suggests that the fibronectin played an important role in the proliferation and migration of the junctional epithelium.

对出生后一周至八十周的小鼠下第一磨牙进行了牙周组织年龄依赖性变化的免疫荧光和免疫电子显微镜研究。 20周时完成的牙槽骨数量逐渐减少，与牙周膜接壤的表面变得不规则，呈波浪状。牙周膜占据的空间随着年龄的增长而宽度减小，这可能是因为新形成的牙骨质沉积在牙根表面。此外，牙周膜和牙龈组织中的细胞也随着年龄的增长而减少。I型和III型胶原蛋白以及纤连蛋白与牙周组织和牙龈组织的纤维紧密相关地分布。 IIl型胶原蛋白被强烈染色，与出生后2周至20周的小鼠的靠近牙槽骨的牙周韧带的纤维束相关。这一事实表明该区域发生了 III 型胶原蛋白的活跃合成。产后30周后的小鼠，交界上皮增殖并沿牙骨质向下迁移至根尖侧。纤连蛋白在迁移的连接上皮的外部基底膜上被强烈染色。这一发现表明纤连蛋白在连接上皮的增殖和迁移中发挥重要作用。