Age-related Change of the Immunohistochemical Localization of Fibronectin and Laminin in association with the Apical Migration of the Junctional Epithelium

纤连蛋白和层粘连蛋白免疫组织化学定位的年龄相关变化与交界上皮的顶端迁移相关

• 批准号: 05671628
• 负责人: SAKAI Takako
• 金额: $ 1.34万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671628/
• 关键词: Fibronectin; Laminin; Junctional epithelium; Aging

Periodontal tissue shows various morphological changes with aging. A typical example of these changes is the apical migration of the junctional epithelium. The distribution of fibronectin (FN) and laminin (LN) was investigated by means of immunofluorescent and immunoelectron microscopic methods in mice to clarify any possible functional roles of these proteins in the progression of apical migration of the junctional epithelium. Apical migration starts in 20-week-old mice, and then progresses with increasing age until the mice reached 80 weeks. In the apical tip of the junctional epithelium, FN was demonstrated in the subepithelial fibrillar matrix, and this phenomenon preceded the progression of apical migration in the junctional epithelium. FN was also demonstrated in association with the stromal side of the focal contact observed between epithelial cells and the basement membrane. Therefore, this focal contact is presumed to be a FN receptor. No apparent relationship between the localization of LN and the migration of the junctional epithelium was observed. These results suggest that the FN provides a provisional matrix for the apical migration of the junctional epithelium. Meanwhile, LN does not appear to play a major role in apical migration.

牙周组织随着​​年龄的增长表现出各种形态变化。这些变化的一个典型例子是交界上皮的顶端迁移。通过免疫荧光和免疫电子显微镜方法研究了小鼠中纤连蛋白（FN）和层粘连蛋白（LN）的分布，以阐明这些蛋白质在交界上皮顶端迁移过程中可能的功能作用。顶端迁移从 20 周龄的小鼠开始，然后随着年龄的增加而进展，直到小鼠长到 80 周。在交界上皮的顶端，FN出现在上皮下纤维基质中，并且这种现象先于交界上皮顶端迁移的进展。 FN 还被证明与上皮细胞和基底膜之间观察到的焦点接触的基质侧相关。因此，该焦点接触被推测为 FN 受体。未观察到 LN 定位与交界上皮迁移之间存在明显关系。这些结果表明 FN 为交界上皮的顶端迁移提供了临时基质。同时，LN 似乎在根尖迁移中并未发挥主要作用。

项目成果

Takako Sakai, Yasuyoshi Ohsaki, Mizuho A.Kido, Mieko Goto, Yoshihiro Terada and Hidetaka Sakai.: "Distribution of Fibronectin and Laminin in the Murine Periodontal Membrane : Possible Functional Roles on the Apical Migration of the Junctional Epithelium"

Takako Sakai、Yasuyoshi Ohsaki、Mizuho A.Kido、Mieko Goto、Yoshihiro Terada 和 Hidetaka Sakai.：“鼠牙周膜中纤连蛋白和层粘连蛋白的分布：对交界上皮根尖迁移的可能功能作用”

Sakai T.,Ohsaki Y.,Kido A.M.,Goto M.,Terada Y.and Sakai H.: "Distribution of Fibroneetin and Laminin in the Murine Periodontal Membrane : Possible Functional Roles on the Apical Migration of the Junctional Epithelium" Archs.oral Biol.(印刷中).

Sakai T.、Ohsaki Y.、Kido A.M.、Goto M.、Terada Y. 和 Sakai H.：“鼠牙周膜中纤连蛋白和层粘连蛋白的分布：对交界上皮根尖迁移的可能功能作用” Archs.oral生物学（正在出版）。

Sakai T., Ohsaki Y., Kido A. M., Goto M., Terada Y. and Sakai H.: "Distribution of Fibronectin and Laminin in the Murine Periodontal Membrane: Possible Functional Roles on the Apical Migration of the Junctional Epithelium" Archs. oral Biol.(印刷中). (1996)

Sakai T.、Ohsaki Y.、Kido A. M.、Goto M.、Terada Y. 和 Sakai H.：“鼠牙周膜中纤连蛋白和层粘连蛋白的分布：对交界上皮根尖迁移的可能功能作用”口腔拱门。生物（正在出版）（1996）。