Development of Hybrid Artificial Graft With Hierarchical Tissue Structure

具有分层组织结构的混合人工移植物的开发

• 批准号: 01570797
• 负责人: MATSUDA Takehisa
• 金额: $ 1.34万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570797/
• 关键词: Hybrid Artificial Graft; Endothelial Cells; Smooth Muscle Cells; 人工血管

Development of Hybrid Artificial Graft ; Fast Nass Culturing & Seeding on Compliant Small-Diameter Artificial Grft. The fast mass culturing of bovine endothelial cells (ECs) were successfully achieved by using collagen microsphere in a spiner flask under mild agitation. The seeded cells adhered and grew well on microspheres. The harvesting by tripsinization resulted in a quite high cell proliferation rate perunit volume. The collected cells were seeded on a compliant small-diameter polyurethane graft overlayered with an artificial basement membrame, which is compose a of type I collagen and dermatame sulfate complex gel. The resultant lumen surface resembles well the morphoqlogy of natural vessel.

混合人工移植物的开发；在顺应的小直径人工移植物上进行快速纳斯培养和播种。通过在旋转烧瓶中温和搅拌下使用胶原微球，成功实现了牛内皮细胞（EC）的快速大量培养。接种的细胞在微球上粘附并生长良好。通过 Tripsinization 收获导致每单位体积的细胞增殖率相当高。将收集的细胞接种到覆盖有人工基底膜的顺应性小直径聚氨酯移植物上，该基底膜由 I 型胶原和硫酸皮肤素复合凝胶组成。所得的管腔表面与天然血管的形态非常相似。

项目成果

Niu, Matsuda & Oka: "Endothelialization on Various Segmented Polyurethones (SPUs) : Cellular Behaviors and Its Substrate Dependency" Trano. Am. Soc. Artif. Intern. Orgamo. 36. 164-167 (1990)

牛松田

Matsuda & Kurumatani: "SurfaceーInduced in vitro Angiogenesis:Surface Property is Determinant of Angiogenesis" Trans.Am.Soc.Artif.Intern.Organs.36. 192-195 (1990)

Matsuda 和 Kurumatani：“表面诱导的体外血管生成：表面特性是血管生成的决定因素”Trans.Am.Soc.Artif.Intern.Organs.36（1990）。

Niu,Matsuda & Oka: "Endothelializaion on Various Segmented Palyurethanes(SPUS): Cellular Behaviors and Its Substrate Dependency" Frans.Am.Soc.Artifi.Intern.Organs.36. 164-167 (1990)

牛松田

Niu,Matsuda & Oka: "In Vitro Model System of Endothelialization at Anastomotic Site: Endothelial Cell Motility on Artificial Substrates" Trans.Am.Soc.Artif.Intern.Organs.36. 757-760 (1990)

牛松田

Matsuda & Kurumatani: "Surface- Induced in Vitro Angiogenesis : Surface Property is Determinant of Angiogenesis" Trans Am. Soc. Artip. Intern. Organ. 36. 192-195 (1990)

松田