Tissue Engineering of Vascular Wall Reconstruction : Fundamentals & Application

血管壁重建的组织工程：基础知识

• 批准号: 07044303
• 负责人: MATSUDA Takehisa
• 金额: $ 2.94万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044303/
• 关键词: Mechanical stress; Hybrid Tissue; Tissue Engineering; Cells; 細胞; 内皮細胞; 機械刺激; 血管壁

The international collaborative study between National Cardiovascular Center and University of Wisconsin have launched to deepen understanding of vascular cell types under mechanical stress field and its potential applications to tissue-engineered and artificial organs such as vascular vessels and hearts. At the National Cardiovascular Center, vascular cell types include endothelial cells and smooth muscle cells have been exposed to hydrodynamic sheer stress and mechanical distension, which have studied focusing cytoskeletal arrangement and phenotype alteration. Hierarchical structured hybrid tissues were constructed and impianted into canine arteries. On the other hand, endothelialization on Milwaukee Heart was achieved.Exchange of information, techniques and methodologies upon mutual visits to respective institutes were very useful fon conducting ongoing projects at both sides.

国家心血管中心和威斯康星大学之间的国际合作研究已启动，以加深对机械应力场下血管细胞类型的了解及其在血管和心脏等组织工程和人造器官中的潜在应用。在国家心血管中心，包括内皮细胞和平滑肌细胞在内的血管细胞类型已暴露于流体动力学剪切应力和机械扩张，这些细胞重点研究了细胞骨架排列和表型改变。构建了分层结构的混合组织并将其植入犬动脉中。另一方面，密尔沃基心脏实现了内皮化。通过互访各自的机构进行信息、技术和方法的交流对于双方正在进行的项目非常有用。

项目成果

H.Kito,T.Matsuda: "Biocompatible Coatings for Luminal and Outer Surfaces of Small-Caliber Artificial Grafts" Journal of Biomedical Material Research. 30. 321-330 (1996)

H.Kito、T.Matsuda：“小口径人工移植物的管腔和外表面的生物相容性涂层”生物医学材料研究杂志。

H.Kito, T.Matsuda: "Biocompatible coatings for Luminal and Outer Surfaces of Small-Caliber Artificial Grafts" Journal of Biomedical Meterials Research. 30. 321-330 (1996)

H.Kito、T.Matsuda：“用于小口径人工移植物的管腔和外表面的生物相容性涂层”《生物医学材料研究杂志》。

J.Hirai, T.Matsuda: "Venous Reconstruction Using Hybrid Vascular Tissue Composed of Vascular Cells and Collagen : Tissue Rageneration Process" Cell Transplantation. 5. 93-105 (1996)

J.Hirai、T.Matsuda：“使用由血管细胞和胶原蛋白组成的混合血管组织进行静脉重建：组织再生过程”细胞移植。

T.Matsuda,T.Sugawara: "Photochimical Protein Fixation on Polymer Surface via Deriovatized Phenyl Azido Group" Langmuir. 11. 2272-2276 (1995)

T.Matsuda、T.Sukawara：“通过衍生化苯基叠氮基团将光化学蛋白质固定在聚合物表面”Langmuir。

K.Katoh,M.Masuda,Y.Kano,Y.Jinguji,K.Fujiwara: "Focal adhesion proteins associated with stress fibers of human fibroblasts." Cell Motil.Cytoskeleton. 31. 177-195 (1995)

K.Katoh、M.Masuda、Y.Kano、Y.Jinguji、K.Fujiwara：“与人成纤维细胞应力纤维相关的焦点粘附蛋白。”