耐冷性に関する形質転換植物の作製

与耐寒性相关的转化植物的创建

基本信息

批准号：
01840027
负责人：
MURATA Norio
金额：
$ 8.64万
依托单位：
National Institute for Basic Biology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B).
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

1. Purpose: Low temperature is one of the important environments which limit the agricultural production. If chilling-sensitive plants of tropical origin can be converted to be more chilling-resistant, the net food production in the world can be much improved. It has been demonstrated that chilling sensitivity of plants is highly correlated with the level of saturation of fatty acids of phosphatidylglycerol (PG), one of the chloroplastic membrane lipids. It is suggested that the level of saturation of PG is under the control of the enzyme, acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase (ATase). The purpose of this research is to convert a chilling-sensitive plant into a chilling-resistant plant, by technique of gene introduction.2. Results: (1) cDNA for ATase from a chilling resistant plant, Arabidopsis, was introduced into tobacco by Ti plasmid binary vector method. Analyses by southern blot, northern blot and Western blot demonstrated that the introduced gene was incorporated into the genome, and expressed, and that the gene product was transported into the chloroplast and processed to become the mature protein. (2) The analysis of the lipids of the tobacco transgenic plant indicated that the level of saturation of fatty acids was changed in PG, but in no other lipids. (3) By comparing the transgenic plants with control plants, it appeared that the transgenic plants were more resistant to chilling. (4) When the cDNA for ATase from a chilling sensitive plant, squash, was introduced to tobacco, the level of saturation of PG and the chilling sensitivity were both increased.3. Conclusion: It is now possible that the saturation of membrane lipids and also chilling sensitivity of plant can be modified by gene introduction.

1。目的：低温是限制农业生产的重要环境之一。如果可以将热带起源的寒冷敏感植物转化为更耐寒，那么世界上的净粮食生产可以得到很大改善。已经证明，植物的寒冷敏感性与磷酸甘油（PG）的脂肪酸（叶绿素膜脂质之一）高度相关。建议PG的饱和水平在酶的控制下，酰基 - 酰基 - 载蛋白）：3-磷酸甘油 - 3-磷酸酰基转移酶（ATASE）。这项研究的目的是通过基因引言技术将对寒冷的植物转化为一种耐寒的植物。2。结果：（1）Ti质粒二进制载体方法将来自耐寒植物的Atase cDNA引入了烟草。通过Southern印迹，Northern印迹和Western印迹分析表明，引入的基因被纳入基因组并表达，并将基因产物转运到叶绿体中并加工成成熟的蛋白质。（2）对烟草转基因植物的脂质的分析表明，PG中脂肪酸的饱和度水平发生了变化，但没有其他脂质。（3）通过将转基因植物与对照植物进行比较，看来转基因植物对寒冷更具抵抗力。（4）当将来自寒冷敏感植物的Atase的cDNA引入烟草中时，PG的饱和度和寒冷的灵敏度都会增加。3。结论：现在有可能通过基因引入来改变膜脂质的饱和以及植物的寒冷敏感性。