Genetic engineering of temperature stress tolerance

温度胁迫耐受性基因工程

基本信息

批准号：
06044233
负责人：
MURATA Norio
金额：
$ 3.39万
依托单位：
Okazaki National Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Dr.Gombos stayd in the National Institute for Basic Biology from September 6th to December 1st, 1995.He, together with Dr.Murata and Dr.Hayashi, studied the photoinhibition of photosystem II at low temperature in Synechocystis sp.PCC 6803.The Synechocystis cells were exposed to various periods of strong light to induce the photoinhibition, which was followed by the recovery of the photosynthetic machinery from the photoinhibited state under dim light at room temperature.It was demonstrated that the photoinhibition was separated into two components ; one was reversible, and the other was irreversible.The reversible component can be explained by the turnover of the D1 protein in the photosystem II complex.The irreversible component had not been well studied, and its molecular mechanism is unknown.In the view point of physiology, the irreversible photoinhibition is deteriorious to the photosynthetic organisms, whereas the reversible photoinhibition provides them with temporal depression of photosynthesis.2.Dr.Okuyama stayd in Biological Research Center from July 14th to July 29th, 1995.He, together with Dr.Gombos and Dr.Szalontai, studied the cryophilicity and the physical phase of membrane lipids of antarctic Haptophyte, Prymnesiophyte strain B.The photosynthesis of the cells of this strain grown 5ﾟC revealed the optimum temperature at 5ﾟC,and the phase transition of the membrane lipids3.Dr.Murata stayd in Biological Research Center from March 10th to March 29th, 1996.He, together with Dr.Gombos and Dr.Hideg, studied the heat tolerance of Synechococcus sp.PCC 7002.The disruption the groEL-beta gene by insertional mutation decreased the viability of this strain after the heat shock.However, the disruption did not affect the heat stability of the photosynthetic activity.These results are likely to suggest that the heat shock protein GroEl-beta is not related to the heat acclimation.appeared below 5ﾟC.

Gombos博士于1995年9月6日至12月1日呆在美国国家基本生物学研究所。温度证明，将光抑制作用分为两个组成部分。 one was reversible, and the other was irreversible.The reversible component can be explained by the turnover of the D1 protein in the photosystem II complex.The irreversible component had not been well studiod, and its molecular mechanism is unknown.In the view point of physiology, the irreversible photoinhibition is delicate to the photosynthetic organisms, whereas the reversible photoinhibition provides them with temporary depression of光合作用。2.dr.okuyama于1995年7月14日至7月29日住在生物学研究中心。膜脂质3.dr.murata的相过渡于1996年3月10日至3月29日留在生物学研究中心。不影响光合作用活性的热稳定性。这些结果可能表明热休克蛋白groel-beta与热适应无关。出现在5°C以下。