Studies on DNA damages in vivo and in vitro by highly sensitive fluorometric analysis

通过高灵敏荧光分析研究体内和体外 DNA 损伤

• 批准号: 01044096
• 负责人: HAYATSU Hikoya
• 金额: $ 5.57万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01044096/
• 关键词: fluorometry; DNA damage; N-nitrosamines; 8-methoxypsoralen; light; 近紫外光; 突然変異; 蛍光光度分析; DNAー本鎖切断; 光; 活性酸素; 遺伝子損傷阻害物質; フォスゲン; 螢光分析法; 細胞内DNA; ショウジョウバエ

DNA damages can be detected with high sensitivity by use of a fluorometric measurement. This method, originally developed by Morgan (the coinvestigator of this project), is based on the change in the fluorescence-intensity of ethidium bromide upon its intercalation into double-stranded DNA. Through our collaboration with Dr. Morgan, the following achievements were made.1. The fluorometric technique has been acquired by the Japanese workers, and the fluorometric measurement system is now established in Okayama University.2. Several new DNA-damaging agents were discovered. They include tetranitromethane, phosgene and N-nitrosamines with light.3. Cross-linking was detected in DNA that had been treated in vitro with 8-methoxypsoralen and UVA, but no cross-linking was found in the DNA of Drosophila lavae that had been treated with these agents in vivo. These observations indicate that DNA damages other than cross-linking must be responsible for the mutagenesis observed in the wing spots of the adult flies obtained from the lavae.

通过使用荧光测量，可以通过高灵敏度检测DNA损伤。这种方法最初是由摩根（该项目的共同评估者）开发的，它基于溴化乙锭的荧光强度变化，其插入到双链DNA中时。通过与摩根博士的合作，取得了以下成就1。荧光技术已由日本工人采集，荧光测量系统现已在冈山大学建立。2。发现了几种新的DNA损害剂。它们包括具有光的四硝基甲烷，磷酸和N-硝基胺3。在用8-甲氧基索拉伦和UVA体外处理的DNA中检测到了交联，但在果蝇乳果的DNA中未发现交联，在体内用这些药物治疗了这些药物。这些观察结果表明，除交联以外的DNA损坏必须负责从薰衣草获得的成年果蝇的翅膀斑点中观察到的诱变。

项目成果

H. Hayatsu: "Dietary inhibitors of mutagenesis and carcinogenesis" Chemoprevention of Cancer, eds. by S. V. Bhide and G. B. Maru, Omega Scientific Publications, New Delhi. 40-50 (1992)

H. Hayatsu：“诱变和致癌的饮食抑制剂”，癌症化学预防，编辑。

T.Aoe,H.Hayatsu et al.: "DNA single-strand breaks induced in vitro by N-nitrosamines with irradiation of near ultraviolet light." Mutation Research,.

T.Aoe,H.Hayatsu 等人：“N-亚硝胺在近紫外光照射下在体外诱导 DNA 单链断裂。”

H. Hayatsu (ed.): CRC Press, FL. Mutagens in Food : Detection and Prevention, 1-286 (1991)

H. Hayatsu（编辑）：CRC Press，佛罗里达州。

H.Tsuchiyama,H.Hayatsu et al.: "Analysis of 2-amino-N^6-hydroxyadenine-induced mutagenesis in phage M13mp2" Mutation Research. 253. 47-54 (1991)

H.Tsuchiyama，H.Hayatsu 等人：“噬菌体 M13mp2 中 2-氨基-N^6-羟基腺嘌呤诱导的诱变分析”突变研究。

T.Negishi,H.Hayatsu et al.: "The genotoxicities in vivo of N-nitrosamines as assayed in the wing spot test and the DNA-repair test of Drosophila melanogaster" Mutation Research. 252. 119-128 (1991)

T.Negishi、H.Hayatsu 等人：“黑腹果蝇翅斑试验和 DNA 修复试验中测定的 N-亚硝胺体内基因毒性”突变研究。