A new method for monitoring mutagenicity in environmental waters

监测环境水域致突变性的新方法

• 批准号: 02557092
• 负责人: HAYATSU Hikoya
• 金额: $ 6.72万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02557092/
• 关键词: Blue rayon; Mutagenicity; River water; Environmental mutagens; Benzo(a)pyrene; ブル-レ-ヨン; 環境水域; 水域汚染; 海水

In this project, we intended to establish a new method for monitoring mutagenicity of environmental waters and have accomplished the intention. Blue rayon is an adsorbents relective for compounds having polycyclic structures with planar surface. This material was invented in 1983 by Hayatsu, the principal investigator of this project, and is a rayon product bearing copper phthalocyanine trisulfonate as ligand. In this project, we hung blue rayon in river waters for 24 hrs, collected the blue rayon, eluted the polycyclic substances that had been adsorbed to the rayon, and the eluted substances were subjected to the mutagenicity assay on Salmonella. We carried out field work with this technique, in Yodo River, Osaka-Kyoto and in the Seto Inland Sea. This work showed that (1) Yodo River was polluted with mutagens of high potency which were then characterized to be polycyclic aromatic amines; and (2) benzo(a)pyrene was detectable at multiple coast sites of the Seto Inland Sea.With these results, we concluded that although semiquantitative, this new technique is a simple and efficient means to monitor contamination of environmental waters with polycyclic mutagenic compounds.

在这个项目中，我们打算建立一种监测环境水体致突变性的新方法，并且已经达到了目的。蓝色人造丝是针对具有平坦表面的多环结构的化合物的吸附剂。该材料由该项目主要研究者早津于1983年发明，是一种以铜酞菁三磺酸盐为配体的人造丝产品。在本项目中，我们将蓝色人造丝悬挂在河水中24小时，收集蓝色人造丝，洗脱出吸附在人造丝上的多环物质，并将洗脱物进行沙门氏菌致突变性测定。我们利用这项技术在淀川、大阪京都和濑户内海进行了实地工作。本研究表明：（1）淀川河受到高效诱变剂的污染，经鉴定其为多环芳香胺； (2) 在濑户内海的多个海岸点均可检测到苯并(a)芘。根据这些结果，我们得出结论，虽然是半定量的，但这种新技术是监测多环诱变化合物对环境水域污染的简单而有效的方法。

项目成果

H.HAYATSU: "Monitoring of food mutagens." Progress in Clinical and Biological Research Vol.340E,Mutation and the Environment,Part E: Environmental Genotoxicity,Risk,and Modulation,Eds.by M.L.Mendelsohn and R.J.Albertini,Wiley-Liss,New York. 169-178 (1990)

H.HAYATSU：“食品诱变剂的监测。”

H. Hayatsu, S. Arimoto and K. Wakabayashi: "Method for separation and detection of heterocyclic amines." Mutagens in Food: Detection and Prevention, ed. by H. Hayatsu, CRC Press, Boca Raton. 101-112 (1992)

H. Hayatsu、S. Arimoto 和 K. Wakabayashi：“杂环胺的分离和检测方法”。

H.HAYATSU: "Cellulosue bearing covalently linked copper phthalocyanine trisulfonate as an adsorbent selective for polycyclic compounds and its use in studies of environmental mutagens and carcinogens." J.Chromatography. 497. 37-56 (1992)

H.HAYATSU：“带有共价连接的铜酞菁三磺酸盐的纤维素作为多环化合物的选择性吸附剂及其在环境诱变剂和致癌物研究中的应用。”

H.Iwado,H.Hayatsu,et al.,: "Mutagenicity and antimutagenicity of airーborne particulates." Mutation Research. 246. 93-102 (1991)

H. Iwado、H. Hayatsu 等，“空气中颗粒物的致突变性和抗致突变性”，246. 93-102 (1991)。

H.IWADO,M.NAITO and H.HAYATSU: "Mutagenicity and antimutagenicity of air-borne particulates." Mutation Res.246. 93-102 (1991)

H.IWADO、M.NAITO 和 H.HAYATSU：“空气中颗粒物的致突变性和抗突变性。”