The Role of Glycosides Formation on the Degradation of Lignin by Wood-Rotting Fungi.

糖苷形成对木材腐烂真菌降解木质素的作用。

基本信息

批准号：
62560179
负责人：
KONDO Ryuichiro
金额：
$ 1.09万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

In order to clarify the role of carbohydrates components on the biodegradation of lignin in wood, the transformation of lignin model compounds and DHP lignin was studied. The results are as follows;(1)(1) The rate of the consumption of the starting lignin model compounds (4-ethylsyringylglycerol- -syringyl ether, veratryl alcohol, vanillyl alcohol and veratrum aldehyde) by wood-rotting fungi (Phanerochaete chrysosporium, Coriolus versicolor and Tyromyces palustris) dependedon the kind of carbohydrate media. The order of the rate of the modelcompounds consumption was, polysaccharide medium(xylan, holocellulose) disaccharide medium (cellobiose ) monosaccharide medium (glucose,xylose).(2) In the polysaccharide and the disaccharide medium, model compounds were transformed into their glycosides by the transglycosy-lation of glycosidase induced by carbohydrates with wood-rotting fungi. In the xylan and birch holocellulose medium, veratryl alcohol was transformed into veratryl -D-xyloside during the early phase of cultivation. In the cellobiose medium, vanillyl alcohol and veratryl alcohol were biotransformed into vanillyl -D-glucoside and veratryl -D-glucoside, respectively.(3) The toxicity of lignin model compounds against fungus was reduced by the formation of their glycosides. The polymerization of phenolic model compounds by laccaseor lignin peroxidase was reduced by the formation of their glycosides.(4) DNP lignin-glucoside was synthesized by an enzymatic method. Incubation of DHP lignin-glucoside with lignin peroxidase / H_2O_2 resulted in partial depolymerization, although treatment of DHP ligninwith them caused polymerization.

为了阐明碳水化合物成分在木质中木质素生物降解中的作用，研究了木质素模型化合物和DHP木质素的转化。 The results are as follows;(1)(1) The rate of the consumption of the starting lignin model compounds (4-ethylsyringylglycerol- -syringyl ether, veratryl alcohol, vanillyl alcohol and veratrum aldehyde) by wood-rotting fungi (Phanerochaete chrysosporium, Coriolus versicolor and Tyromyces palustris) dependedon the kind of碳水化合物培养基。模型倒数消耗速率的顺序是，多糖培养基（木糖，全纤维素）二糖培养基（纤维纤维）单糖培养基（葡萄糖，木糖）。多糖和二糖培养基中的多糖培养基中的模型通过其糖剂通过糖苷的糖化液转化为糖苷，并通过糖苷转化为糖果，通过糖剂转化为透明度的糖果液，并将其转化为糖果。木质真菌。在Xylan和桦木全纤维素培养基中，在培养的早期阶段将veratryl醇转化为veratryl -d -xyloside。在纤维酶培养基中，分别将香草醇和蔬菜醇生物转化为香丁酯-D-葡萄糖苷和veratryl -D-葡萄糖苷。（3）通过形成糖苷的形成，降低了木质素模型化合物对真菌的毒性的毒性。通过形成其糖苷的形成，通过漆蛋白过氧化物酶对酚类模型化合物的聚合作用。（4）通过酶促方法合成DNP木质素 - 葡萄糖苷。将DHP木质素 - 葡萄糖苷与木质素过氧化物酶 / H_2O_2孵育导致部分解聚，尽管对DHP木质素的处理并引起聚合。