Study on the enzymatic bleaching of kraft pulp

牛皮纸浆酶法漂白的研究

• 批准号: 04660191
• 负责人: KONDO Ryuichiro
• 金额: $ 1.28万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660191/
• 关键词: Enzyme bleaching; Kraft pulp; Phanerochaete sordida; Brightness; Manganese peroxidase; Phanerochaete sordida; マンガンペルオキシターゼ; 非塩素系漂白; 木材腐朽菌; 白色腐朽菌; バイオブリーチング; ダイオキシン; リグニン分解酵素

To Clarify the role of the excreted extracellular enzymes during long-term incubation in a pulp biobleaching system with white-rot fungi, we have developed a cultivation system using a membrane filter which can prevent the direct contact between hyphae and kraft pulp, but allows extracellular enzymes to attack the kraft pulp. Phanerochaete sordida YK-624 brightened the pulp 21.4points to 54.0% brightness after 5-day in vitro treatment, which was significantly higher than phanerochaete chrysosporium and Coriolus versicolor did during 7-day treatment. These results indicate that cell-free, membrane-filtered components from the in vitro bleaching system are capable of delignifying unbleached kraft pulp. Obvious candidates for filterable reagents capable of delignifying and bleaching kraft pulp are peroxidase and phenoloxidase proteins. Secreted manganese peroxidase activity in the filterable components was substantial during the fungus YK-624 in vitro bleaching. A positive correlation between the level of manganese peroxidase and brightening of the pulp was observed.

为了阐明在果肉生物漂白系统与白rot真菌中长期孵育期间排泄的细胞外酶的作用，我们使用膜过滤器开发了一种培养系统，该膜过滤器可以防止菌丝和牛皮纸之间的直接接触，但允许细胞外牙髓之间的直接接触酶以攻击牛皮纸浆。 5天的体外处理后，Phanerochaete sordida YK-624将纸浆21.4点亮至54.0％的亮度，在7天治疗期间，这显着高于Phanerochaete chrysosporium和Coriolus versicolor。这些结果表明，来自体外漂白系统的无细胞，膜过滤组件能够划定未漂白的牛皮纸浆。明显的候选物用于脱氧和漂白的牛皮纸果肉的可过滤试剂是过氧化物酶和苯酚氧化酶蛋白。在真菌YK-624的体外漂白过程中，可过滤成分中的分泌锰过氧化物酶活性是很大的。观察到锰过氧化物酶的水平与果肉的亮度之间存在正相关。

项目成果

近藤,隆一郎: "In Vitro Bleaching of Hardwood Kraft Pulp by Extracellular Engymes Excreted from white Ret Fungi in a Cultivation System Using a Membrane Filter" Applied and Environmental Microbiology. 60. (1994)

Ryuichiro Kondo：“在使用膜过滤器的培养系统中，通过从白色 Ret 真菌中排出的细胞外酶对硬木牛皮纸浆进行体外漂白”，应用和环境微生物学 60。（1994 年）。

近藤隆一郎: "In Vitro Bleaching of Hardwood Kraft Pulp by Extracellular Enzymes Excreted from White Rot Fungi in a Cultivation System Using a Membrane Filter." Applied and Environmental Microbiology. 60. (1994)

Ryuichiro Kondo：“使用膜过滤器在培养系统中通过白腐真菌分泌的细胞外酶对硬木牛皮纸浆进行体外漂白。”（1994 年）。

Ryuichiro KONDO: "In Vitro Bleaching of Hardwood Kraft Pulp by Extracellular Enzymes Excreted from White Rot Fungi in a Cultivation System Using a membrane Filter." Applied and Environmental Microbiology. volume 60, issue 3. (1994)

Ryuichiro KONDO：“在使用膜过滤器的培养系统中，通过白腐真菌分泌的细胞外酶对硬木牛皮纸浆进行体外漂白。”