In vitro treatment of dioxins by enzymes from white-rot fungus.

白腐真菌酶对二恶英的体外处理。

• 批准号: 10460076
• 负责人: KONDO Ryuichiro
• 金额: $ 7.81万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10460076/
• 关键词: Dioxin; White rot fungi; Bioremediation; in vitro; lignin; Basidiomycetes; 環境汚染; ダイオキシン類; エーテル結合開裂; ダイオキシン分解酵素; エーテル結合; 2,7-DCDD

The decrease of 2,7-dichlorodibenzo-p-dioxin (diCDD)-recovery was observed by the incubation with the culture fluid from white-rot fungus Ceriporia sp.strain MZ-340. The decrease of recovery reached 40% of 50uM during 180min period. The recovery of diCDD in the boiled control was higher than 90%. Not recovered diCDD was remained in the water layer, and it was retrievable by treating reaction mixture by acid, alkali or surfactant. Such diCDD-trapping activity was lost by a protease treatment and heating.We characterized the diCDD-trapping activity of the extracellular protein solution (EPs) from the fungus. The pH optimum of the activity was obtained at pH 2.7. More than 80% of the maximal activity was observed within a range of 25-45℃. This activity decreased dramatically as the incubation temperature increased. In the effects of organic solvent concentrations, the activity was disappeared by the addition of 5.0% of acetone or 12.5% of N,N-dimethylformamide, respectively.The substrate specificity was examined on the trapping-activity. Less or no trapping activity was observed for dibenzofuran (DF), biphenyl (BP), dibenzothiophene, carbazole, fluorene, diphenyl ether and phenanthrene. The trapping-activity for triCDD, diCDF, diCBP and anthracene showed over 50%, and that for DD, monoCDD and tetraCDD were lower than 40%, when their rates were expressed as percentages of the activity for diCDD.These results showed that the specificity of the trapping activity depends on the hydrophobicity of the substrate.Furthermore, the concentrated EPs could trap even octaCDD.

在180分钟期间，培养液的培养液降低了2,7-二氯二苯甲酸杆菌素（DICDD）的降低沸腾的对照中的RECDD高于90％。在2.7的pH值中获得了超过80％的最大活性。 N-二甲基甲酰胺，分别在诱捕活性上检查底物的特异性。低于40％s表明，捕获活性的特异性取决于底物的疏水性。浓缩的EPS甚至可能捕获八粒。

项目成果

近藤隆一郎: "ダイオキシンとキノコ"APAST. 36. 10-15 (2000)

近藤龙一郎：“二恶英和蘑菇”APAST 36. 10-15 (2000)。

Ryuichiro, Kondo: "Dioxin and mushroom"APAST. 36. 10-15 (2000)

近藤龙一郎：“二恶英和蘑菇”APAST。

近藤隆一郎: "微生物によるダイオキシン類の分解"環境科学会誌. 12. 462-471 (1999)

Ryuichiro Kondo：“微生物分解二恶英”环境科学学会杂志 12. 462-471 (1999)。

Ryuichiro, Kondo: "Degradation of dioxines by microorganisms"Kankyo Kagaku Kaishi. 12. 462-471 (1999)

近藤龙一郎：“微生物对二恶英的降解”Kankyo Kagaku Kaishi。