Bioremediation of pollutants by xenobiotic ligninolytic fungi

异生木质素真菌对污染物的生物修复

• 批准号: 06453177
• 负责人: KONDO Ryuichiro
• 金额: $ 3.97万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06453177/
• 关键词: Basidiomycetes; White-rot fungi; PCB; Bioremediation; Ligninolytic enzymes; Dioxines; リグニン生分解; 木材腐朽菌; ダイオキシン

Methods of degradation of dioxins and PCBs by white rot fungi were developed. Degradation of a mixture of total 10 kinds of tetra to octa of polychlorodibenzo-p-dioxins (PCDDs) and polychlorodibenzofurans (PCDFs), which were chlorinated 2-, 3-, 7- and 8-positions of the molecules, by white rot fungi Phanerochaete sordida YK-624 was studied in a stationary low nitrogen medium. The degradation rates of PCDDs and PCDFs were ca.40 (tetrachloro-) -76 (hexachloro-) % and 45 (tetrachloro-) -70 (hexachloro) %, respectively. Of PCDDs, metabolites of 2,3,7,8-tetrachloro-, and 1,2,3,4,6,7,8,9-octa-, dibenzo-p-dioxin by P.sordida YK-624 were studied and 4,5-dichloro-1,2-benzoquinone and 3,4,5,6-tetrachloro-1,2-benzoquinone were confirmed as oxidatitive cleavage products of dibenzodioxin nuclus. PCBs were first hydroxylated and then C-C bonds were cleaved to form benzoic acid derivatives. These results suggested that the white rot fungi are substantially able to degrade both PCDDs/PCDFs and PCBs. In conclusion, this is the first report on the degradation of highly chlorinated PCDDs and PCDFs by microorganisms.

开发了白腐真菌降解二恶英和多氯联苯的方法。白腐真菌降解分子 2、3、7、8 位共 10 种四至八种多氯二苯并二恶英 (PCDD) 和多氯二苯并呋喃 (PCDF) 混合物在固定低氮培养基中研究了 Phanerochaete sordida YK-624。 PCDDs和PCDFs的降解率分别为约40(四氯-)-76(六氯)%和45(四氯-)-70(六氯)%。在 PCDD 中，研究了 P.sordida YK-624 的 2,3,7,8-四氯-和 1,2,3,4,6,7,8,9-八-，二苯并-对-二恶英的代谢物4,5-二氯-1,2-苯醌和3,4,5,6-四氯-1,2-苯醌被确认为氧化裂解产物二苯并二恶英核。 PCB首先被羟基化，然后C-C键断裂形成苯甲酸衍生物。这些结果表明白腐真菌基本上能够降解 PCDD/PCDF 和 PCB。总之，这是第一篇关于微生物降解高氯化 PCDD 和 PCDF 的报告。

项目成果

R. Kondo et al: "Degradation of Polychlorinated bibenzo-p-dioxins and Polychlorinated Dibenzofurans by Wlute Rot Fungus YK-624." Appl. Environ. Microbiology. 62(発表予定). (1996)

R. Kondo 等人：“Wute Rot Fungus YK-624 降解多氯二苯并二恶英”（Appl Environ Microbiology）（1996 年）。

R.Kondo et al.: "Degradation of Polychlorinated Dibenzo-p-dioxines and Polychlorinated Dibenzofurans by White-rot Fungus Phanerochaete sordida YK-624." Appl.Enviro.Microbiol.(to be published).

R.Kondo 等人：“白腐真菌 Phanerochaete sordida YK-624 降解多氯二苯并-对二恶英和多氯二苯并呋喃。”