In vivo production and hematopoietic activity of interleukin 3 (IL-3) in mice

小鼠体内白细胞介素 3 (IL-3) 的产生和造血活性

基本信息

批准号：
61570595
负责人：
KANAMARU Akihisa
金额：
$ 1.34万
依托单位：
Hyogo College of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

We have demonstrated a high level of interleukin 3(IL-3) in the serum of mice with graft-versus-host disease(GVH-D).The level of IL-3 rised as the GVH-D became severer.The level of this factor was measured by the method of either erythroid colony-formation from 14 day-fetal mouse liver cells or^3H-TdR uptake in FDC-P2 cells which are IL-3 dependent cell line.The inhibitory activity was detected in the serum by the method using FDC-P2 cells but not erythroid colony-forming technique.However,the specificity or physiological role of this activity was obscure.The possibility that the hematopoietic activity of the serum might be due to a factor induced by endotoxin was excluded by the experiments with endotoxin non-responder C3H/Hej mice,in which the activity was elevated as much as the control mice when suffering from GVH-D.The production of IL-3 was observed in liquid culture media from bone marrow,spleen and lymph node cells in GVH-D mice.The in vivo effect of IL-3 was confirmed in mice injected with the serum from polycytemic GVH-D mice, in which serum erythropoietin level was reduced into undetectable range.Total numbers of BFU-E,CFU-C and CFU-Mix were remarkably increased in spleens of the mice 3 days after the injection.IL-3 in the serum stimulated the proliferation and differentiation of the hematopoietic progenitors in mice. Consequently,it plays and important role in hematopoiseis in vivo as well as in vitro.

我们已经证明，患有移植物抗宿主病（GVH-D）的小鼠血清中白细胞介素3（IL-3）水平较高。随着GVH-D的加重，IL-3的水平也随之升高。该因子通过来自14天胎儿小鼠肝细胞的红细胞集落形成的方法或FDC-P2细胞(其是IL-3依赖性细胞系)中的^3H-TdR摄取的方法来测量。使用FDC-P2细胞的方法而非红系集落形成技术检测到血清中的抑制活性。然而，该活性的特异性或生理作用尚不清楚。血清的造血活性可能是由于内毒素无反应C3H/Hej小鼠实验排除了内毒素诱导因素，其活性与患GVH-D的对照小鼠一样升高。IL-3的产生在 GVH-D 小鼠的骨髓、脾脏和淋巴结细胞的液体培养基中观察到 IL-3 的体内作用在注射多细胞 GVH-D 小鼠血清的小鼠中得到证实，其中血清促红细胞生成素水平为减少至不可检测范围。注射后第3天，小鼠脾脏中BFU-E、CFU-C和CFU-Mix总数明显增加。血清中IL-3刺激小鼠造血祖细胞的增殖和分化。因此，它在体内和体外的造血中发挥着重要作用。