Role of complement regulatory proteins and adherent factors on the differentiation from the stem cells in paroxysmal nocturnal hemoglobinuria

补体调节蛋白和粘附因子在阵发性睡眠性血红蛋白尿症干细胞分化中的作用

• 批准号: 03671200
• 负责人: KANAMARU Akihisa
• 金额: $ 1.28万
• 依托单位: Hyogo college of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03671200/
• 关键词: PNH; stem cell; GPI anchor proteins; DAF; HRF20; HRF20; PNH; GPIアンカ-; 細胞接着因子; 造血幹細胞

Expressions of complement regulatory protein, HRF20 have been investigated on the cell membrances of hematopoietic stem cells and their progeny cells in bone marrows from patients with paroxysmal nocturnal hemoglobinuria(PNH). PNH nonphagocytic mononuclear marrow cells were labeled with anti-HRF20 monoclonal antibody and subsequently with FITC-conjugated secondary antibody and then sorted into HRF20-negative and -positive fractions with a FACS, followed by the culturing with them in methylcellulose. CFU-GM colonies and BFU-E bursts were formed in the cultures 10 to 14 days later. These colonies/bursts were plucked up and their progeny cells were analyzed with respect to expression of HRF20. The expression was negative on all of the progeny cells from HRF20-negative progenitors, while the cells from HRF20-positive progenitors revealed both negative and positive expressions of HRF20 on their cell membranes.

补体调节蛋白 HRF20 在阵发性睡眠性血红蛋白尿症 (PNH) 患者骨髓中的造血干细胞及其子代细胞的细胞膜上的表达进行了研究。 PNH非吞噬性单核骨髓细胞用抗HRF20单克隆抗体标记，随后用FITC缀合的二抗标记，然后用FACS分选为HRF20阴性和阳性级分，随后将它们在甲基纤维素中培养。 10 至 14 天后，培养物中形成 CFU-GM 集落和 BFU-E 爆发。拾取这些集落/爆发并分析其后代细胞的 HRF20 表达。来自HRF20阴性祖细胞的所有子代细胞的表达均为阴性，而来自HRF20阳性祖细胞的细胞在其细胞膜上显示出HRF20的阴性和阳性表达。

项目成果

西村 朋子: "PNH血球膜における補体制御因子発現の多様性について" 臨床血液(Abstrart). 32. 13l7 (1991)

Tomoko Nishimura：“PNH 血细胞膜中补体调节因子表达的多样性”《临床血液》（摘要）32. 13l7 (1991)。