Coordinating mechanisms of prostaglandins and vasotocin in the control of ovulation-oviposition

前列腺素和催产素控制排卵产卵的协调机制

基本信息

批准号：
61560301
负责人：
SHIMADA Kiyoshi
金额：
$ 1.28万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Uterine electromyographic activity (EMG),plasma concentrations of prostaglandin(PG) F and E, and arginine vasotocin(AVT) were simultaneously measured in relation to PGE_2-induced premature oviposition and to indomethacin-blocked oviposition in chickens. The administration of indomethacin (5 mg, im) 3 hr before the predicted time of oviposition delayed egg lay by 8-14 hr, whereas control hens laid eggs at the predicted time. In indomethacin-treated hens increases in uterine EMG activity and plasma concentrations of PGF,PGE,and AVT were not observed at the expected time of oviposition, whereas a marked increase in plasma PGF and AVT occurred during spontaneous oviposition . The intrauterine administration of PGE_2(1<micrn>g) 3 hr before predicted oviposition induced premature egg lay within 5 min. Premature oviposition was accompanied by significant elevations in both uterine EMG activity and plasma levels of AVT. Changes in plasma levels of PGF and PGE were not observed. When premature oviposition was induced, significant increases in EMG activity and plasma concentrations of PGF and AVT were observed at the expected time of oviposition in the absence of an egg in the uterus. The administration of PGE_2 at the predicted time of oviposition in hens pretreated with indomethacin induced oviposition which was accompanied by a significant increase in plasma AVT levels. The results suggest that AVT release is stimulated by uterine contractility in chickens.

子宫肌电图活性（EMG），前列腺素（PG）F和E的血浆浓度以及精氨酸血管蛋白（AVT）同时测量与PGE_2诱导的过早外卵和鸡肉中的吲哚美辛蛋白阻滞性卵巢相对于PGE_2诱导的早产。在预测的产卵卵延迟延迟8-14小时之前，给予吲哚美辛（5 mg，im）3小时，而对照鸡在预测的时间产生鸡蛋。在吲哚美辛治疗的母鸡中，子宫EMG活性的增加和PGF，PGE和AVT的血浆浓度在预期的产卵时间没有观察到，而在自发性卵形期间，血浆PGF和AVT显着增加。在预测产卵诱导的过早卵之前，宫内给药PGE_2（1 <mincn> g）3小时。过早的产卵伴随着子宫EMG活性和血浆AVT水平的显着升高。未观察到PGF和PGE血浆水平的变化。当诱导过早的排卵时，在子宫中没有卵的情况下，在产卵的预期时间观察到EMG活性显着增加，PGF和AVT的血浆浓度显着增加。在预测用吲哚美辛诱导的产卵的母鸡的预测时间内给予PGE_2，伴随着血浆AVT水平的显着升高。结果表明，鸡的子宫收缩力刺激了AVT释放。