Molecular and cellular basis of mosquito olfactory attraction to hay infusion for enhanced vector surveillance and control

蚊子对干草浸液的嗅觉吸引力的分子和细胞基础，以加强病媒监测和控制

• 批准号: 10648584
• 负责人: Conor James McMeniman
• 金额: $ 24.12万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-18 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10648584
• 关键词: Adhesives; Aedes; Afferent Neurons; African; Analytical Chemistry; Arboviruses; Behavior; Behavioral; Behavioral Assay; Biological Assay; Blood; Botanicals; Cessation of life; Chemicals; Chemoreceptors; Constitution; Constitutional; Culicidae; Cynodon; Data; Dengue; Desiccation; Development; Disease Vectors; Engineering; Female; Fermentation; Formulation; Future; Genetic; Goals; Grant; Gravid; Heterozygote; Human; Infusion procedures; Laboratories; Ligands; Mediating; Methods; Molecular; Molecular Target; Neurons; Olfactory Pathways; Oviposition; Partner in relationship; Pathway interactions; Physiological; Play; Population; Population Control; Population Dynamics; Population Surveillance; Puerto Rico; Research; Resistance; Reverse engineering; Role; Searching Behavior; Sensory; Series; Site; Source; Testing; Water; Yellow Fever; ZIKA; behavior test; cell type; egg; feeding; field study; improved; insight; loss of function; man; mutant; neural; neural circuit; neurophysiology; novel; olfactory sensory neurons; preference; pregnant; receptor; response; tool; urban setting; vector; volatile organic compound

The mosquito Aedes aegypti is a prolific disease vector that thrives in urban environments. Its explosive population dynamics are driven by the ability of female Ae. aegypti to seek out standing pools of water to lay their desiccation-resistant eggs. Of note, females are attracted to the scent of decomposing botanical matter in water, but only after mating and blood-feeding. In particular, gravid female Ae. aegypti are highly attracted to the scent of fermenting African Bermuda grass hay in water, which has been exploited for mosquito trapping strategies in the field that aim to lure this major disease vector to its death during the act of egg laying. We propose to apply integrative methods to define the molecular and cellular basis of Ae. aegypti olfactory attraction to hay infusion which will catalyze identification of olfactory circuitry mediating oviposition site search and novel oviposition attractants for this disease vector. To elucidate candidate chemoreceptors and associated neurons implicated in mosquito olfactory attraction to hay infusion, we will test the role of different chemoreceptor classes using loss-of-function genetics and olfactory sensory neuron populations by selectively disrupting neural activity using cell-type specific neuronal silencing. In parallel, we aim to reverse engineer attractive blends of oviposition attractants mimicking the scent of hay infusion by leveraging chemical analysis of infusion headspace combined with neural activity assays that compare olfactory sensitivity and response amplitudes of non-gravid and gravid mosquitoes to conserved volatile organic compounds found in this this natural lure. These aims will fundamentally improve our understanding of the chemosensory basis of Ae. aegypti oviposition site search behavior. From a translational perspective, this research will reveal new molecular targets in the Ae. aegypti olfactory system to modulate mosquito egg laying behavior and identify novel synthetic formulations of oviposition attractants for enhanced population surveillance and control of this globally important disease vector.

蚊子埃及埃及是一种多产的疾病媒介，在城市环境中繁衍生息。它的爆炸性 人口动态是由女性AE的能力驱动的。埃及寻找站立的水池 他们的耐药性鸡蛋。值得注意的是，女性被分解的植物性物质所吸引 水，但仅在交配和喂血后。特别是妊娠女性AE。埃及很吸引 在水中发酵非洲百慕大草干草的气味，该草被用于蚊子捕获 该领域的策略旨在吸引这种主要的疾病载体在产卵行为期间死亡。我们 建议应用整合方法来定义AE的分子和细胞基础。埃及嗅觉 吸引干草输注，这将催化介导产卵场搜索的嗅觉电路的识别 以及该疾病载体的新型产卵吸引剂。阐明候选化学感受器和 与蚊子嗅觉吸引与干草输注有关的相关神经元，我们将测试不同的作用 使用功能丧失遗传学和嗅觉感觉神经元种群的化学感受器类别通过有选择性 使用细胞型特异性神经元沉默破坏神经活动。同时，我们的目标是反向工程 产卵的有吸引力的混合物吸引者通过利用化学分析模仿干草输注的气味 输注顶空的结合与比较嗅觉敏感性和反应的神经活动测定法 在此发现的无砂粒和妊娠蚊子振幅对保守的挥发性有机化合物 自然诱饵。这些目标从根本上可以提高我们对AE化学感应基础的理解。 埃及产卵场搜索行为。从转化的角度来看，这项研究将揭示新的 AE中的分子靶标。埃及嗅觉系统以调节蚊子卵子的行为并识别 产卵吸引剂的新型合成配方，以增强人口监视和控制 全球重要的疾病载体。