Molecular studies on incubation behavior and the application

孵化行为的分子研究及应用

基本信息

批准号：
07044190
负责人：
SHIMADA Kiyoshi
金额：
$ 8.58万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044190/
关键词：
prolactin incubation behavior chicken mRNA prolactin receptor pituitary hypothalamus PRL mRNA VIP Receptor GnRH antisense PRLmRNA GHmRNA VIP GRF cyclic AMP PRL-Rc recombinant PRL

项目摘要

Now commercial White Leghorn strain chicken dose not express incubation behavior, but Japanese native strain chicken and turkey hens still express incubation behavior. Therefore, in these birds, the incubation behavior lowers productivity of meat and egg laying, causing a lot of economic loss in poulty industries. Therefore, the poupose of present study is to reveal molecular mechanism of the incubation behavior and to establish the application for the removal of incubation behavior of these birds.Turkey and Gifu-jidori hens still express incubation behavior. During the reproductive cycle in these birds, levels of plasma prolactin (PRL) and pituitary PRL mRNA increased during the incubation period but these levels were low in the laying period. These results suggest that PRL may regulate induction and maintaining of incubation behavior.Using perifusion culture of the pituitary and cell culture of pituitary cells, we demonstrated that VIP induces PRL synthesis and release in the pituita … More ry gland via cAMP cascade. We cloned chicken VIP receptor mRNA.There were no significant difference in the distribution of brain PRL receptor mRNA between chabohen displaying expression of incubation behavior and White Leghorn hens without expression of incubation behavior. In addition, during reproductive cycle in chabo hen the levels of brain PRL receptor mRNA did not change.Effects of remobal of newly hatched chicks on incubation behavior were studied in the chabo hens. Those hens whose chicks were removed from the nest for 24 hr displayd again incubation behavior but those hens whose chicks were removed from the nest for 72 hr did not show incubation behavior again. The plasma levels of PRL decreased in all hens whose chicks were removed from the nest for 24 and 72hr. This results suggested that other factor may regulate the maintain of incubation behavior.The administrations of VIP antibody into turkey and chicken hens markedly reduced plasma levels of PRL and inhibited incubation behavior. Less

现在，商业白色Leghorn菌株鸡剂量不表达孵化行为，但是日本的本地菌株鸡和火鸡母鸡仍表达孵化行为。因此，在这些鸟类中，孵化行为降低了肉类和卵的生产率，从而在家禽行业造成了很多经济损失。因此，本研究的概述是揭示孵化行为的分子机制，并确定去除这些鸟类的孵育行为的应用。Turkey和gifu-jidori母鸡仍然表达孵化行为。在这些鸟类的复制循环中，血浆催乳素（PRL）和垂体PRL mRNA的水平在孵育期间增加，但在铺设期间这些水平较低。这些结果表明，PRL可能调节诱导和维持孵育行为。使用垂体细胞的垂体和细胞培养的周期培养，我们证明了VIP会影响PRL的合成和垂体中的释放……通过CAMP Cascade，更多的Ry腺。我们克隆了鸡肉贵族受体mRNA。脑PRL受体mRNA的分布没有显着差异，在没有孵化行为表达的情况下显示出孵化行为表达的Chabohen和白色Leghorn母鸡的表达。此外，在Chabo Hen的生殖周期中，脑PRL受体mRNA的水平没有改变。在Chabo母鸡中，新近孵化的雏鸡对孵育行为的雷神对孵化行为进行了研究。那些将小鸡从巢中取出的母鸡再次显示出孵化行为，但是那些从巢中除去鸡的鸡并未再次表现出孵化行为。所有鸡从巢中除去24和72小时的母鸡的PRL水平均降低。该结果表明，其他因素可能调节孵化行为的维持。将VIP抗体施用到土耳其和鸡肉母鸡中明显降低了PRL的血浆水平并抑制了孵化行为。较少的