Induction of Hormone Autonomous Suspension Culture derived from Leaf Explants of Sugar Beets

甜菜叶外植体激素自主悬浮培养物的诱导

基本信息

批准号：
60540428
负责人：
MASUDA Hiroshi
金额：
$ 0.83万
依托单位：
Obihiro University of Agriculture and Veterinary Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60540428/
关键词：
Habituated cultured cells 2,4D Requiring cultured cells Cell cluster formation 不形根形成

项目摘要

Hormone autonomous callus was initiated reproducibly on MS medium with 0.25mg/l BA as a sole plant hormone (AI medium) from 4-week-old leaf explants of sugar beet, whereas 2,4-D requiring callus were initiated on AI medium to which 0.1mg/l 2,4-D was added. On the other hand, when leaf explants were inoculated into liquid AI medium and cultured on a reciprocal shaker, single cells began to be released from the edge of explants at 6 days after inoculation of explants, followed by active release. When single cells obtained were transferred to fresh liquid MS medium without plant hormone, they could olivide and grow autonomously, giving rise to hormone autonomous suspension cultures. Whereas single cells released in MS medium without BA seldom formed cell cluster in MS medium with or without BA. Accordingly only single cells released from the leaf explants treated with BA could form cell clusters, but in this case, the formation of clusters from single cells proceeded regardless of the presence and alsence of BA. On the other hand, when single cells were cultured in the conditioning medium of hormone autonomous suspension cultured cells, cell clusters, differed from autonomous cell clusters were formed and adventive root from them was observed to be formed.

激素自主愈伤组织是在含有 0.25mg/l BA 作为唯一植物激素（AI 培养基）的 MS 培养基上从 4 周龄的甜菜叶外植体中重复启动的，而需要 2,4-D 的愈伤组织是在 AI 培养基上启动的。添加0.1mg/l 2,4-D。另一方面，当将叶外植体接种到液体AI培养基中并在往复摇床上培养时，在接种外植体后6天，单细胞开始从外植体边缘释放，随后主动释放。当获得的单细胞转移到不含植物激素的新鲜液体MS培养基中时，它们可以橄榄化并自主生长，产生激素自主悬浮培养物。而在不含 BA 的 MS 培养基中释放的单细胞在含有或不含 BA 的 MS 培养基中很少形成细胞簇。因此，只有从用BA处理的叶外植体中释放的单个细胞才能形成细胞簇，但在这种情况下，无论BA是否存在，单个细胞的簇形成都会继续进行。另一方面，当在激素自主悬浮培养细胞的条件培养基中培养单细胞时，形成与自主细胞簇不同的细胞簇，并且观察到由它们形成不定根。