Novel Carrot Embryo-forming System.

新型胡萝卜胚胎形成系统。

基本信息

批准号：
05640723
负责人：
MASUDA Hiroshi
金额：
$ 1.34万
依托单位：
Obihiro University of Agriculture and Veterinary Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

We have developed a novel embryo-forming system in which carrot somatic embryogenesis takes place in a one-stage process from epidermal cells in the absence of 2,4-D,provided hypocotyl explants are incubated in a liquid MS medium containing 2,4-D for 12-24 hours. In addition, in this system, somatic embryos develop directly via formation of cluster cells by repeating division of organized epidermal cells with adhesion to explants, without dedifferentiation to callus and suspension cultured cells. Somatic embryogenesis occurred directly from carrot epidermal cells was observed using scanning electron microscopy.Epidermal cells peeled off from parent tissues were present as compressed cells and then expanded to become tubelar cells. Subsequently, tubular cells were regularly and horizontally divided to from linear cluster cells and then these cluster cells divided vertically to become enlargment. Swelling structure were formed by irregular and oblique division at places on linear cluster cells and then continued to divide to form small proembryos. Some cells which comprised surface of swelling structure and proembryos expanded to every direction to become covered on surface of structure. These expanding cells made probably put a layr of surface of structure and furthermore piled up cell layr to form from swelling to globular-stage embryo via small proembryo. These expanding cells were formed on surface of heart-stage embryo.

我们已经开发了一种新型的胚胎形成系统，在没有2,4-D的情况下，在一个阶段的过程中，在一个阶段的过程中，在一个阶段的过程中进行了荷叶州体细胞胚，前提是在含有2,4--的液体MS培养基中孵育下的苯丙基外植体D持续12-24小时。此外，在该系统中，体细胞胚胎通过重复有组织的表皮细胞对外植体的有组织的表皮细胞直接形成而直接发展，而无需将愈伤组织和悬浮培养的细胞放置分化。使用扫描电子显微镜直接从胡萝卜表皮细胞中直接发生了体细胞胚发生。表皮细胞从母体组织中剥离，以压缩细胞为压缩细胞，然后膨胀以成为小管细胞。随后，将管状细胞定期和水平从线性簇细胞分开，然后这些簇细胞垂直划分以扩大。在线性簇细胞上的位置不规则和倾斜的划分形成肿胀结构，然后继续分裂以形成小的前晶。一些包含肿胀结构和前脊的表面的细胞扩展到每个方向，以覆盖在结构表面上。这些扩展的细胞可能使结构的表面呈外行，然后通过小pro果从肿胀到球阶段的胚胎形成细胞层。这些扩展的细胞在心脏阶段胚胎的表面形成。