Development of A New Method to Investigate Cell Migration in the Developing Central Nervous System

开发一种研究发育中中枢神经系统细胞迁移的新方法

• 批准号: 09680740
• 负责人: TAMAMAKI Nobuaki
• 金额: $ 2.05万
• 依托单位: Department of Anatomy, Fukui Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680740/
• 关键词: Migration; Migration speed; Olfactory Buld; Thymin-dimer; UV-light; Fiber Optic Cable; チミンダイマー; 紫外線照射; 色素性乾皮症; ミュータントマウス; 光ファイバー

We have devised a novel method to study cell migration in vitro and in vivo. The new method involves UV-irradiating cells through a fiber optic cable and subsequently identifying irradiated cells by virtue of the formation of thymine-dimer in the nuclei. We applied the new method to investigate migration of neuronal precursor cells in the rostral migratory stream in the neonatal rat olfactory bulb. In vitro, UV-irradiation for one second through the fiber optic cable allowed formation of enough thymine-dimers to be detected immunohistochemically after 6 hours of incubation. A significant proportion of irradiated cells continued to migrate in the same direction at the same speed as before irradiation. There was no significant difference in the cell-migration distance at 6 hours with or without the UV-irradiation in vitro. We applied this UV-thymine-dimer labeling method to study cell migration in the olfactory bulb in vivo, With this method, we could find that a small number of cells migrated also caudally. Three times more cells in the SV of the olfactory bulb migrated rostrally, but there was also a significant extent of caudally directed cell migration. The new method also allowed us to measure the speed of cell-migration, which was estimated to be about 70 ? m/hr to the rostral direction and 37 ? m/hr to the caudal direction, at maximum.

我们已经设计了一种新的方法来研究细胞在体外和体内的迁移。新方法涉及通过光纤电缆的紫外线呈紫外线细胞，随后通过核中胸腺胺的形成来鉴定受辐照的细胞。我们应用了一种新方法来研究新生大鼠嗅球的鼻迁移流中神经元前体细胞的迁移。在体外，通过光纤电缆进行一秒钟，可以在6小时孵育6小时后形成足够的胸腺素。大量的辐照细胞继续以与辐照前相同的速度沿相同的方向迁移。如果有或没有体外的紫外线辐照，则在6小时时细胞迁移距离没有显着差异。我们将这种UV-甲基二聚体标记方法应用于体内嗅球中的细胞迁移，使用这种方法，我们可以发现少数细胞也持续迁移。嗅球SV中的细胞多三倍，但也有很大的定向细胞迁移。新方法还使我们能够测量细胞迁移的速度，估计约为70？ M/hr到延髓方向，37？ m/hr到尾端，最大。

项目成果

Tamamaki,N.et al.: "Origin and route of tangentially migrating neurons in the developing neocorteical intermediate zone." Journal of Neuroscience. 17. 8313-8323 (1997)

Tamamaki,N.et al.：“发育中的新皮质中间区切向迁移神经元的起源和路线。”

Nobuaki Tamamaki, Yoshihiko Sugimoto, Kiyoji Tanaka, and Rumiko Takauji: "Tangential cell migration in the neocortex monitored by marking nuclei with UV-irradiation via a fiber optic cable." Development. (submitted). (1999)

Nobuaki Tamamaki、Yoshihiko Sugimoto、Kiyoji Tanaka 和 Rumiko Takauji：“通过光纤电缆用紫外线照射标记细胞核来监测新皮质中的切向细胞迁移。”

Tamamaki N.et al.: "Tangential cell migration in the neocortex monitored by marking nuclei with UV-irradiation via a fiber optic cable." Development. submitted.

Tamamaki N.等人：“通过光纤电缆用紫外线照射标记细胞核来监测新皮质中的切向细胞迁移。”

Tamamaki N.et al.: "The development of afferent fiber lamination in the infrapyramidal Blade of the rat dentate gyrus." J.Comp.Neurol.in press.

Tamamaki N.等人：“大鼠齿状回锥体下叶片中传入纤维层压的发展。”