Functions of neocortical neurons originating in the ganglionic eminence

起源于神经节隆起的新皮质神经元的功能

• 批准号: 12680731
• 负责人: TAMAMAKI Nobuaki
• 金额: $ 2.37万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680731/
• 关键词: Ganglionic eminence; inhibitory neuron; cell migration; guidance cue; Sema3A; Sema3F; 大脳基低核原基; Ganglionic eminence; inhibitory neuron; cell migration; guidance cue; Sema3A; Sema3F; 大脳基低核原基

Basically all the neocortical neurons have been believed to originate in the neocortical ventricular zone. However, the investigator ofthis project found that the GABAergic neurons in the neocortex originate in the ganglionic eminence and migrate to the neocortex at embryonic stages. On the way to migrate from the ganglionic eminence to the neocortex, we could not found any scaffold for the guidance of the tangential cell migration. Instead we found neuropilin-1 and 2 on the Dil labeled tangentially migrating cells. Ligands of neuropilin-1 and -2, Sema3A and 3F were expressed in the primordium of striatum and seemed to repel the neuropilin-1 and -2 positive cells to the neocortex, respectively. In addition, the neuropili-1 positive cells and the neuropilin-2 positive cells were segregated into the IZ and into the cortical plate by Sema3A expression in the VZ and Sema3F expression in the cortical plate, respectively. Neuropilin-1 and Dlx2 positive cells seemed to become the interneurons in the cortical plate. Neuropilin-2 and Dlx2 positive cells seemed to become the pioneer neurons andoligodendrocyte in the IZ. Some of the pioneer neurons seemedto remain in the white matter as white matter neurons which connectneocortices.

基本上，所有新皮质神经元都被认为起源于新皮质心室区域。然而，该项目的研究者发现，新皮层中的GABA能神经元起源于神经节卓越，并在胚胎阶段迁移到新皮层。在从神经节的隆起到新皮层的途中，我们找不到任何用于切向细胞迁移的指导的支架。取而代之的是，我们在被标记为切向迁移细胞的DIL上发现了Neuropilin-1和2。 Neuropilin -1和-2，Sema3a和3F的配体在纹状体的原始中表达，似乎分别将Neuropilin -1和-2阳性细胞驱除到新皮层中。此外，分别在VZ中通过SEMA3A表达将Neuropili-1阳性细胞和Neuropilin-2阳性细胞分离到IZ和皮质板中，并分别在皮质板中的SEMA3F表达。 Neuropilin-1和DLX2阳性细胞似乎已成为皮质板中的中间神经元。 Neuropilin-2和DLX2阳性细胞似乎已成为IZ中的先驱神经元和果胶胶质细胞。一些先锋神经元似乎作为白质神经元连接神经元的神经元。