Research on the intermediate progenitors of GABAergic neurons found in the subventricular zone of the mouse neocortex

小鼠新皮质脑室下区 GABA 能神经元中间祖细胞的研究

• 批准号: 20300121
• 负责人: TAMAMAKI Nobuaki
• 金额: $ 12.65万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20300121/
• 关键词: 再生医学; 神経科学; 大脳皮質; 神経前駆細胞; 神経細胞; intermediate progenitor; GABA; neocortex; proliferation; array analysis

In the embryonic neocortex, neuronal precursors are generated in the ventricular zone(VZ) and accumulate in the cortical plate. Recently, the subventricular zone(SVZ) of the embryonic neocortex was recognized as an additional neurogenic site for both principal excitatory neurons and GABAergic inhibitory neurons. To gain insight into the neurogenesis of GABAergic neurons in the SVZ, we investigated the characteristics of intermediate progenitors of GABAergic neurons(IPGNs) in mouse neocortex by immunohistochemistry, immunocytochemistry, single-cell RT-PCR, and single-cell array analysis. IPGNs were identified by their expression of some neuronal and cell cycle markers. Moreover, we investigated the origins of the neocortical IPGNs by Cre-loxP fate mapping in transgenic mice and the transduction of part of the telencephalic VZ by Cre-reporter plasmids, and found them in the medial and lateral ganglionic eminence. Therefore they must migrate tangentially within the telencephalon to reach the neocortex. Cell-lineage analysis by simple-retrovirus transduction revealed that the neocortical IPGNs self-renew and give rise to a small number of neocortical GABAergic neurons and a large number of granule and periglomerular cells in the olfactory bulb. IPGNs are maintained in the neocortex and may act as progenitors for adult neurogenesis.

在胚胎新皮层中，神经元前体是在心室区域（VZ）中产生的，并积聚在皮质板上。最近，胚胎新皮层的室心（SVZ）被认为是主要兴奋性神经元和GABA能抑制神经元的附加神经源部位。为了深入了解SVZ中GABA能神经元的神经发生，我们研究了通过免疫组织化学，免疫细胞化学，单细胞质RT-PCR和单细胞ELL驱动器分析，通过免疫组织化学，免疫组织化学，免疫组织化学，免疫组织化学，IMPCN和单细胞化学分析。 IPGN通过某些神经元和细胞周期标记的表达来识别。此外，我们研究了转基因小鼠中Cre-loxp命运图的新皮质IPGN的起源，以及Cre-Reporter质粒对部分触及脑VZ的一部分转导，并在中间和外侧神经节明显中找到它们。因此，它们必须在远导脑内切向迁移以达到新皮层。通过简单逆转录病毒转导的细胞训练分析表明，新皮层IPGN自我更新并引起少量的新皮层GABA能神经元，以及嗅觉小块中的大量颗粒和周围细胞。 IPGN保持在新皮层中，可以充当成人神经发生的祖细胞。

项目成果

Method for single-cell microarray analysis and application to gene-expression profiling of GABAergic neuron progenitors

• DOI: 10.1016/j.neures.2007.12.011
• 发表时间: 2008-04-01
• 期刊: NEUROSCIENCE RESEARCH
• 影响因子: 2.9
• 作者: Esumi, Shigeyuki;Wu, Sheng-Xi;Tamamaki, Nobuald
• 通讯作者: Tamamaki, Nobuald

Draxin, a repulsive axon guidance protein, is involved in hippocampal development

• DOI: 10.1016/j.neures.2009.09.1710
• 发表时间: 2010-01-01
• 期刊: NEUROSCIENCE RESEARCH
• 影响因子: 2.9
• 作者: Zhang, Sanbing;Su, Yuhong;Tanaka, Hideaki
• 通讯作者: Tanaka, Hideaki

Noradrenergic excitation of a subpopulation of GABAergic cells in the basolateral amygdala via both activation of nonselective cationic conductance and supression of resting K+conductance : A study using GAD67-GFP knock-in mice

通过非选择性阳离子电导的激活和静息 K 电导的抑制来激发基底外侧杏仁核中 GABA 能细胞亚群的去甲肾上腺素能：使用 GAD67-GFP 敲入小鼠的研究

• 发表时间: 2008
• 期刊: Neuroscience
• 影响因子: 3.3
• 作者: Kaneko K;Tamamaki N;Owada H;Kakizaki T;Kume N;Totsuka M;Yamamoto T;Yawo H;Yagi T;Obata K;Yanagawa Y.
• 通讯作者: Yanagawa Y.

Excess Salt Causes Cerebral Neuronal Apoptosis and Inflammation in Stroke-Prone Hypertensive Rats Through Angiotensin II-Induced NADPH Oxidase Activation

• DOI: 10.1161/strokeaha.108.517284
• 发表时间: 2008-11-01
• 期刊: STROKE
• 影响因子: 8.3
• 作者: Yamamoto, Eiichiro;Tamamaki, Nobuaki;Kim-Mitsuyama, Shokei
• 通讯作者: Kim-Mitsuyama, Shokei

Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice

• DOI: 10.1016/j.jchemneu.2009.04.003
• 发表时间: 2009-09
• 期刊: Journal of Chemical Neuroanatomy
• 影响因子: 2.8
• 作者: Yayun Wang;Yan‐Yan Wei;Jing Huang;Wei Wang;Nobuaiki Tamamaki;Yun-Qing Li;Sheng-Xi Wu