Genetic characterization of a sex-, chromosome-, and tissue-specific mechanism of gene expression regulation

基因表达调控的性别、染色体和组织特异性机制的遗传特征

• 批准号: 515890405
• 负责人: Professor Dr. John Parsch
• 金额: --
• 依托单位: Department Biologie II (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/515890405?language=en
• 关键词: Genetic; characterization; sex; chromosome; tissue

Similar to mammals, the fruit fly Drosophila melanogaster has heteromorphic sex chromosomes, with males having one X and one Y chromosome, and females having two X chromosomes. In male somatic tissues, expression of the single X chromosome is up-regulated through the well-known process of dosage compensation. In the male germline, expression of the X chromosome is suppressed through a mechanism that is not yet understood. To gain insight into this process, we will take advantage of two independent mutant lines of D. melanogaster that are deficient in X suppression in the male germline. Through whole-genome sequencing of recombinant males, we will map the mutations and associated genes that affect X suppression. We will also sequence the testis transcriptome of mutant males to characterize the effect of the mutations on the expression of X-linked genes. Finally, we will perform functional experiments to validate candidate genes and determine their role in regulating gene expression. The results will advance our understanding of how gene expression is regulated in a sex-, chromosome-, and tissue-specific manner.

与哺乳动物类似，果蝇果蝇具有异形性染色体，雄性有一条 X 和一条 Y 染色体，雌性有两条 X 染色体。在男性体细胞组织中，单个 X 染色体的表达通过众所周知的剂量补偿过程上调。在雄性种系中，X 染色体的表达通过一种尚不清楚的机制受到抑制。为了深入了解这一过程，我们将利用两个独立的黑腹果蝇突变系，它们在雄性种系中缺乏 X 抑制。通过重组雄性的全基因组测序，我们将绘制影响 X 抑制的突变和相关基因。我们还将对突变男性的睾丸转录组进行测序，以表征突变对 X 连锁基因表达的影响。最后，我们将进行功能实验来验证候选基因并确定它们在调节基因表达中的作用。这些结果将增进我们对基因表达如何以性别、染色体和组织特异性方式进行调节的理解。