Molecular characterization of the ocular microbiome in healthy adults

健康成人眼部微生物组的分子特征

• 批准号: 10661148
• 负责人: Sapna S Gangaputra
• 金额: $ 63万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10661148
• 关键词: Acinetobacter; Adult; Anterior; Anterior eyeball segment structure; Bacteria; Biological; Biomass; Bradyrhizobium; Clinical; Collaborations; Corynebacterium; Culture Media; Data; Disease; Elderly; Environment; Epithelium; Exposure to; Gene Expression; Genes; Genetic Transcription; Genus staphylococcus; Homeostasis; Human; Human Microbiome; Human body; Immune; Immune response; Immune system; Inferior; Knowledge; Metagenomics; Methods; Methylobacterium; Microbe; Molecular; Mucous Membrane; Ophthalmology; Outcome Study; Pathway interactions; Population; Propionibacterium; Pseudomonas; Publishing; Research Personnel; Ribosomal RNA; Role; Sampling; Seasons; Sex Differences; Skin; Sphingomonas; Streptococcus; Structure; Swab; Taxon; Virus; age group; analytical tool; anterior chamber; bacteria classification; cohort; conjunctiva; cost effective; eye chamber; fungus; immunoregulation; metagenome; metagenomic sequencing; metatranscriptome; metatranscriptomics; microbial; microbial community; microbiome; microorganism; middle age; multidisciplinary; next generation sequencing; novel; ocular microbiome; ocular surface; prospective; sample collection; young adult

SUMMARY The ocular surface is constantly exposed to the environment and is inhabited by several microbes. The most commonly used methods for ocular surface microbiome characterization consist of traditional microbiological culturing and, more recently, next-generation sequencing. Culture-based methods provide information on absolute abundances but are limited in identifying only fast-growing microorganisms on culture media and a large number of nonculturable microbes. Recent 16S rRNA marker gene sequencing studies provide the most extensive and diverse characterization of the ocular microbiome. This data suggests the existence of the resident ocular microbiome; however, little is known about the core microbiome constituents of the anterior segment in a healthy population. Furthermore, the optimal method of sample collection that will yield the most representative results remains unclear. This study aims to implement whole-metagenome sequencing (WMS) and metatranscriptomics sequencing methods optimized for low-biomass samples to characterize the ocular microbiome inhabiting the anterior segment of the eye and their interaction with the immune system in healthy adults. In this project, we will establish a large human cohort (n=500) and will prospectively collect serial samples from inferior and superior conjunctival fornix from healthy adults for a period of 1 year. In addition to characterizing the core ocular microbiome in healthy adults, we will examine seasonal changes in the diversity and enrichment of specific microbial taxon (bacteria, fungi, and viruses); ocular microbiome differences between sexes and different age groups (young adults, middle age, and older adults). The metatranscriptomics data will enable us to profile active microbial pathways (bacteria, fungi, and virus) and host epithelial (conjunctival) immune response for underpinning associations between the microbiome and ocular immune modulation. The outcomes of this study will contribute towards understanding the core microbial constituents in the anterior segment and their role in immune interactions to maintain homeostasis.

概括 眼表不断暴露在环境中，并且栖息着多种微生物。 眼表微生物组表征最常用的方法包括 传统的微生物培养和最近的下一代测序。以文化为基础 方法提供有关绝对丰度的信息，但仅限于识别快速生长的 培养基上的微生物和大量不可培养的微生物。最近的16S rRNA 标记基因测序研究提供了最广泛和多样化的特征 眼部微生物群。该数据表明存在常驻眼部微生物组；然而， 人们对健康人群眼前节的核心微生物组成分知之甚少。 此外，将产生最具代表性的结果的最佳样本采集方法 仍不清楚。本研究旨在实施全宏基因组测序（WMS）和 针对低生物量样品优化的宏转录组测序方法，以表征 居住在眼前节的眼部微生物群及其与免疫系统的相互作用 健康成年人的系统。在这个项目中，我们将建立一个大型人类队列（n = 500）并将 前瞻性地从健康成人的下结膜和上结膜穹窿收集系列样本 为期1年。除了表征健康成年人的核心眼部微生物群外，我们还 将检查特定微生物分类群（细菌、 真菌和病毒）；性别和不同年龄组（年轻人）之间的眼部微生物组差异 成人、中年人和老年人）。宏转录组学数据将使我们能够分析活性 微生物途径（细菌、真菌和病毒）和宿主上皮（结膜）免疫反应 微生物组和眼部免疫调节之间的基础关联。结果 这项研究将有助于了解前部的核心微生物成分 片段及其在维持体内平衡的免疫相互作用中的作用。