Regulatory Roles and Networks of the Whole Set of Transcription Factors in Escherichia coli

大肠杆菌中整套转录因子的调控作用和网络

• 批准号: 21241047
• 负责人: ISHIHAMA Akira
• 金额: $ 28.79万
• 依托单位: Hosei University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21241047/
• 关键词: ゲノム制御; 転写制御; 転写因子; 大腸菌; ストレス応答; RNAポリメラーゼ; プロモーター; 制御ネットワーク; Genomic SELEX; 転写調節; 環境応答; 転写制御ネットワーク; RNAボリメラーゼ; Phenotype microarray; 原核生物

The genome expression is determined by controlling the distribution of a limited number of RNA polymerase within the genome through interaction with transcription factors. The aim of this research was to identify the regulatory role of all 300 transcription factors in the model prokaryote, Escherichia coli.1) We developed the in vitro Genomic SELEX screening system and determined the regulation target genes for all 300 transcription factors.2) We also developed the in vivo NIP-chip system and determined the intracellular distribution of transcription factors within the E. coli genome.3) The regulation targets thus predicted were examined by measuring their expression in the presence and absence of transcription factors.4) We determined the intracellular levels of all 300 transcription factors by using quantitative immune-blot analysis.5) Taking all the results together, I have proposed :(1) the list of regulation targets for all 300 transcription factors ; and(2) the interaction network of 300 transcription factors, altogether forming complex hierarchic regulation networks.

基因组表达是通过控制有限数量的RNA聚合酶在基因组中通过与转录因子的相互作用来确定的。 The aim of this research was to identify the regulatory role of all 300 transcription factors in the model prokaryote, Escherichia coli.1) We developed the in vitro Genomic SELEX screening system and determined the regulation target genes for all 300 transcription factors.2) We also developed the in vivo NIP-chip system and determined the intracellular distribution of transcription factors within the E. coli genome.3) The regulation targets thus predicted were examined by 4）我们通过使用定量免疫印迹分析确定了所有300个转录因子的细胞内水平。5）我提出了所有结果：（1）所有300个转录因子的调节目标列表； （2）300个转录因子的相互作用网络，完全形成复杂的层次调节网络。

项目成果

Regulatory role of MlrA in transcription activation of csgD, the master regulator of biofilm formation in Escherichia coli

• DOI: 10.1111/j.1574-6968.2010.02112.x
• 发表时间: 2010-11-01
• 期刊: FEMS MICROBIOLOGY LETTERS
• 影响因子: 2.1
• 作者: Ogasawara, Hiroshi;Yamamoto, Kaneyoshi;Ishihama, Akira
• 通讯作者: Ishihama, Akira

Regulation of the E.coli csgD gene encoding the master regulator of biofilm formation : Interplay between multiple transcription factors 0925023GC004 Micro-Nano Mechatronics Human Sci.

编码生物膜形成主调节因子的大肠杆菌 csgD 基因的调节：多个转录因子之间的相互作用 0925023GC004 微纳机电一体化人类科学。

• 发表时间: 2009
• 作者: Ogasawara;H.;Kori;A.;Yamada;K.;Yamamoto;K.;Ishihama;A.
• 通讯作者: A.

大腸菌全tRNA遺伝子発現プロファイルの解析

大肠杆菌总 tRNA 基因表达谱分析

• 发表时间: 2009
• 作者: Mitani;N.;Ma;J.F.;長谷川喬彬・梅林章・五浜明・前田理久
• 通讯作者: 長谷川喬彬・梅林章・五浜明・前田理久

大腸菌転写因子DeoRの新規標的遺伝子群の同定と制御機能解明

大肠杆菌转录因子DeoR新靶基因的鉴定和调控功能的阐明

• 发表时间: 2009
• 作者: Ma;J.F.;Yamaji;N.;Mitani;N.;中川史之・島田友裕・石田雄士・石浜明
• 通讯作者: 中川史之・島田友裕・石田雄士・石浜明

大腸菌プロモーターのin vitroにおける網羅的解析

大肠杆菌启动子的综合体外分析

• 发表时间: 2011
• 作者: 鈴木香織;山中幸;島田友裕;石浜明;山本兼由
• 通讯作者: 山本兼由