MOLECULAR ANATOMY OF TRANSCRIPTION APPARATUS

转录装置的分子解剖学

• 批准号: 08044107
• 负责人: ISHIHAMA Akira
• 金额: $ 1.34万
• 依托单位: NATIONAL INSTITUTE OF GENETICS
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044107/
• 关键词: RNA polymerase ;; transcription factor :; DNA-protein interaction; protein-protein interaction ;; molecular assembly ;; structure-function relation ;; Escherichia coli ;; transcription regulation; 蛋白質-蛋白質相互作用

RNA polymerase plays a key role in gene transcription. Transcription apparatus in both prokaryotes and eukaryotes are composed of RNA polymerase and one or more of transcription factors. The promoter selectivity of prokaryotic RNA polymerases is modulated in two steps of molecular communications, i.e., the first step with one of sigma factors and the second step with one or more of the transcription factors. In order to understand the regulatory mechanisms of promoter selectivity of the RNA polymerase, protein-protein contact sites between subunits and between subunits and tranascription factors have been mapped by both genetic and biochemical methods. Results indicated that : the concentration and activity of seven different sigma factrs involved in the first-step differentiation in Escherichia coli vary depending on cell growth conditions ; there are about 100 different species of the second-step factors in E.coli, which can be classified into four groups depending on the contact sites on RNA polymerase subunits ; more than two factors are able to interact with the RNA polymerase independently.

RNA聚合酶在基因转录中起关键作用。原核生物和真核生物中的转录设备均由RNA聚合酶和一个或多个转录因子组成。核酸RNA聚合酶的启动子选择性以分子通信的两个步骤进行调节，即，具有一个或多个转录因子的第二步，第二步是一个或第二个步骤。为了了解RNA聚合酶的启动子选择性的调节机制，遗传学和生化方法都绘制了亚基之间以及亚基和三膜法之间的蛋白质 - 蛋白质接触位点。结果表明：七种不同的sigma factrs的浓度和活性参与大肠杆菌的第一步分化，取决于细胞生长条件；大约有大约100种不同的第二步因子，可以根据RNA聚合酶亚基上的接触位点分为四组；超过两个因素能够独立与RNA聚合酶相互作用。

项目成果

Jair, K.-W.: "Ambidextrous transcription activation by SosS : Requirement for the C-terminal domain of the RNA polymerase alpha subunit at a subset of superoxide inducible genes." Mol.Microbiol.19(2). 307-314 (1996)

Jair, K.-W.：“SosS 的双手灵巧的转录激活：对超氧化物诱导基因子集的 RNA 聚合酶 α 亚基的 C 端结构域的要求。”

Arisimovitch, I.: "Transcription activation by the bacteriophage Mu Mor protein requires the C-terminal regions of both alpha and sigma^<70> subunits of Escherichiacoli RNA polymerase." J.Biol.Chem.271. 32343-32348 (1996)

Arisimovitch, I.：“噬菌体 Mu Mor 蛋白的转录激活需要大肠杆菌 RNA 聚合酶的 α 和 sigma^<70> 亚基的 C 末端区域。”

Giladi, H.: "Identification of an UP element within the IHF binding site at the P_L1-P_L2 tandem promoter of bacteriophage lambda." J.Mol.Biol.260. 484-491 (1996)

Giladi, H.：“鉴定 lambda 噬菌体 P_L1-P_L2 串联启动子的 IHF 结合位点内的 UP 元件。”

Giladi,H.: "Identification of an UP element within the IHF binding site at the P_L1-P_L2 tandem promoter of bacteriophase λ." J.Mol.Biol.260. 484-491 (1996)

Giladi, H.：“细菌相 λ 的 P_L1-P_L2 串联启动子内的 UP 元件的鉴定。J.Mol.Biol.260 (1996)。”

Tang,Y.: "Upstream interactions at the lambda P_<RM> promoter are sequence-nonspecific and activate the promoter to a lesser extent than an introduced UP element of an rRNA promoter." J.Bacteriol.178・23. 6945-6951 (1996)

Tang, Y.：“lambda P_<RM> 启动子的上游相互作用是序列非特异性的，并且启动子的激活程度低于引入的 rRNA 启动子的 UP 元件。”J.Bacteriol.178·23。 (1996)