Dynamics of signal transduction in the system of organogenesis and regeneration

器官发生和再生系统中信号转导的动力学

基本信息

批准号：
13044002
负责人：
YASUGI Sadao
金额：
$ 327.3万
依托单位：
Tokyo Metropolitan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2005
项目状态：
已结题

项目摘要

In 2005, the networks of gene expressions in the formation of many organ systems have been analyzed. In planarians, two more noggin-like genes were identified and role of these genes in the establishment of body axis was demonstrated (Agata). In amphibians, the function of MA35, a newly found gene involved in the heart formation, was examined. Also Wnt signaling cascade in the early stages of development was extensively studied. These investigations opened a new horizon of organogenesis research in amphibians (Asashima). Co-factors necessary for the transcription of TbxS were identified. Knockdown of some of them in zebrafish showed close relationship between transcriptional control and cytoskeletal system (Ogura). In the course of cartilage formation during the limb development, Hox genes determine the positional characteristics of pre- cartilage cell mass. It was also found that ephrin-b1 and EphB2 function as downstream cell adhesion factors (Kuroiwa). In Drosophila visual system, the suppression of JAK/STAT signal resulted in the shift of lamina neuron to medulla neuron. The JAK/STAT and Notch signals are suggested to regulate multistep differentiation of neurons from the neural stem cells (Tabata). Wnt5a was found to regulate cell differentiation of the epithelium in the glandular stomach of the chicken embryo. Overexpression and suppression of this gene resulted in the overexrpession and/or ectopic expression of some region-specific marker genes of the epithelium. (Yasugi)

2005年，已经分析了许多器官系统形成中基因表达的网络。在平面人中，鉴定了另外两个类似Noggin的基因，并证明了这些基因在身体轴建立中的作用（AGATA）。在两栖动物中，检查了与心脏形成有关的新发现的基因MA35的功能。在开发的早期阶段，WNT信号级联也得到了广泛的研究。这些研究开辟了两栖动物（Asashima）的器官发生研究的新视野。确定了TBXS转录所需的co因子。斑马鱼中其中一些的敲低显示了转录控制与细胞骨架系统（Ogura）之间的密切关系。在肢体发育过程中软骨形成过程中，HOX基因确定了软骨细胞质量的位置特征。还发现，埃弗林-B1和EPHB2充当下游细胞粘附因子（kuroiwa）。在果蝇的视觉系统中，JAK/STAT信号的抑制导致椎板神经元转移到髓质神经元。建议提出JAK/STAT和NOTCH信号来调节神经干细胞的神经元的多疗法分化（TABATA）。发现Wnt5a调节鸡胚胎腺体胃上皮的细胞分化。该基因的过表达和抑制导致上皮某些区域特异性标记基因的过度剥落和/或异位表达。（Yasugi）