Study on the mesenchymal factors regulating gene expression in the epithelium

间充质因子调控上皮基因表达的研究

基本信息

批准号：
06454687
负责人：
YASUGI Sadao
金额：
$ 4.42万
依托单位：
TOKYO METROPOLITAN UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

We have investigated the molecular nature of mesenchymal factors regulating the gene expression in the epithelilal cells during the development of the digestive tract in the chikcen embryo. First we cleary demonstrated that the differentiation and morphogenesis of the epithelium of the digestive organs are regulated by some factors of the mesenchyme. At the same time the ability to respond to such action of the mesenchyme resided in the epithelium is definitively important. The regional difference of the epithelial responsiveness seemed to be related to the expression patterns of several genes encoding transcriptional factors and sonic hedgehog (morphogen). These genes may define the regional characters. The expreimental embryological methods revealed that the expression of these genes is also under regulation of the mesenchyme. For example, CdxA,which is expressed solely in the posterior gut epithelium is induced in the anterior gut epithelium by the mesenchyme of the intestine. As for the molecular nature of the mesenchymal influences, we postulated that some growth factors are involved in the induction. Especially, bone morphogenetic proteins are very plausible candidates, since the genes encoding these proteins are expressed in the regions which has strong inducing avtivity at the developmental stages relevant to the induction. These results altogether indicate the importance of epithelial-mesenchymal interactions in the organogenesis and give us the valuable information on the molecular mechanisms of the interactions.

我们研究了鸡胚胎消化道发育过程中调节上皮细胞基因表达的间充质因子的分子性质。首先我们清楚地证明了消化器官上皮的分化和形态发生受到间充质某些因子的调节。同时，对上皮细胞中间充质的这种作用做出反应的能力绝对是重要的。上皮反应性的区域差异似乎与编码转录因子和音刺猬（形态发生素）的几个基因的表达模式有关。这些基因可以定义区域特征。实验胚胎学方法表明这些基因的表达也受到间充质的调节。例如，仅在后肠上皮中表达的CdxA是由肠间充质在前肠上皮中诱导的。至于间充质影响的分子性质，我们假设一些生长因子参与了诱导。特别地，骨形态发生蛋白是非常合理的候选者，因为编码这些蛋白的基因在与诱导相关的发育阶段具有强诱导活性的区域中表达。这些结果共同表明了上皮间质相互作用在器官发生中的重要性，并为我们提供了有关相互作用分子机制的有价值的信息。