Function analysis of soxgenes in the development and differentiation of digestion

soxgenes在消化发育和分化中的功能分析

基本信息

批准号：
09044233
负责人：
YASUGI Sadao
金额：
$ 4.99万
依托单位：
TOKYO METOROPOLITAN UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

The present study intended to clarify the functions of Sox genes, which recently become known to play important roles in various processes of organogenesis, in the development and differentiation of vertebrate digestive organs.Sox genes encode transcription factors having HMG domain and more than 20 genes have been cloned so far. We paid special attention to cSox2 and cSox21, which are expressed in the gut epithelium of chicken embryo. We analyzed in the present study their expression patterns, regulation of these genes by the mesenchyme, and functional analysis using gene transduction methods. Both genes are expressed in the epithelium of anterior digestive organs such as esophagus, proventriculus and gizzard. Their expression ceased when embryonic morphogenesis and differentiation come to end. Their posterior boundary of expression coincided with the anterior boundary o CdxA, a gene specifically expressed in the intestinal epithelium. Moreover, their expression was very low in the epithelium of newly formed proventricular glands.Expression of cSox2 and cSox21 was shown to be regulated by the mesenchyme. When the intestinal epithelium was cultivated with gizzard mesenchyme some epithelial cells became positive to cSox2 and cSox21. Conversely, the expression of both genes was suppressed in stomach epithelium cultivated with intestinal mesenchyme.Introduction and forced expression of cSox2 and cSox21 into intestinal epithelium never resulted in the suppression of CdxA, suggesting that some partner molecules are necessary for the suppression of target genes by cSox2 and cSox21 gene production.Thus, in the present study, possible involvement of cSox2 and cSox21 in the organogenesis of the digestive organs was demonstrated. In addition, the basic data for further analysis of their function were accumulated.

本研究旨在阐明 Sox 基因的功能，最近人们知道 Sox 基因在器官发生的各种过程、脊椎动物消化器官的发育和分化中发挥着重要作用。Sox 基因编码具有 HMG 结构域的转录因子，超过 20 个基因具有 HMG 结构域。至今已被克隆。我们特别关注在鸡胚肠上皮中表达的cSox2和cSox21。我们在本研究中分析了它们的表达模式、间充质对这些基因的调节以及使用基因转导方法的功能分析。这两种基因均在食道、腺胃和肌胃等前消化器官的上皮中表达。当胚胎形态发生和分化结束时，它们的表达停止。它们的表达后边界与 CdxA（一种在肠上皮中特异性表达的基因）的前边界一致。此外，它们在新形成的前室腺上皮中的表达非常低。cSox2和cSox21的表达被证明受到间充质的调节。当用砂囊间充质培养肠上皮时，一些上皮细胞对 cSox2 和 cSox21 呈阳性。相反，在用肠间充质培养的胃上皮中，这两个基因的表达均受到抑制。将cSox2和cSox21引入肠上皮并强制表达从未导致CdxA的抑制，这表明一些伙伴分子对于抑制靶基因是必需的cSox2 和 cSox21 基因的产生。因此，在本研究中，cSox2 和 cSox21 可能参与消化器官的器官发生被证明。此外，还为进一步分析其功能积累了基础数据。