Function analysis of soxgenes in the development and differentiation of digestion

soxgenes在消化发育和分化中的功能分析

基本信息

批准号：
09044233
负责人：
YASUGI Sadao
金额：
$ 4.99万
依托单位：
TOKYO METOROPOLITAN UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

The present study intended to clarify the functions of Sox genes, which recently become known to play important roles in various processes of organogenesis, in the development and differentiation of vertebrate digestive organs.Sox genes encode transcription factors having HMG domain and more than 20 genes have been cloned so far. We paid special attention to cSox2 and cSox21, which are expressed in the gut epithelium of chicken embryo. We analyzed in the present study their expression patterns, regulation of these genes by the mesenchyme, and functional analysis using gene transduction methods. Both genes are expressed in the epithelium of anterior digestive organs such as esophagus, proventriculus and gizzard. Their expression ceased when embryonic morphogenesis and differentiation come to end. Their posterior boundary of expression coincided with the anterior boundary o CdxA, a gene specifically expressed in the intestinal epithelium. Moreover, their expression was very low in the epithelium of newly formed proventricular glands.Expression of cSox2 and cSox21 was shown to be regulated by the mesenchyme. When the intestinal epithelium was cultivated with gizzard mesenchyme some epithelial cells became positive to cSox2 and cSox21. Conversely, the expression of both genes was suppressed in stomach epithelium cultivated with intestinal mesenchyme.Introduction and forced expression of cSox2 and cSox21 into intestinal epithelium never resulted in the suppression of CdxA, suggesting that some partner molecules are necessary for the suppression of target genes by cSox2 and cSox21 gene production.Thus, in the present study, possible involvement of cSox2 and cSox21 in the organogenesis of the digestive organs was demonstrated. In addition, the basic data for further analysis of their function were accumulated.

本研究旨在阐明Sox基因的功能，该基因最近已知在器官发生的各种过程中起着重要作用，在脊椎动物消化器官的发展和分化中。SOX基因编码具有HMG结构域和20个基因的转录因子，到目前为止已经克隆了20个基因。我们特别注意CSOX2和CSOX21，这些CSOX2和CSOX21在鸡肉胚胎的肠道上皮中表达。我们在本研究中分析了它们的表达模式，通过间充质对这些基因的调节以及使用基因转导方法的功能分析。这两个基因均在前消化器官的上皮（例如食道，proventriculus和gizzard）上表达。当胚胎形态发生和分化结束时，它们的表达停止了。它们的表达的后边界与前边界O CDXA相吻合，该基因在肠上皮中特别表达。此外，它们在新形成的预脑腺上皮中的表达非常低。cSOX2和CSOX21的表达表明由间充质调节。当用牙齿间质培养肠上皮时，一些上皮细胞变得呈CSOX2和CSOX21阳性。相反，两种基因的表达在用肠道间质培养的胃皮细胞中抑制。引入和强迫CSOX2和CSOX21在肠上皮上的表达中，从未导致CDXA抑制CDXA，这表明某些伴侣分子是对CSSOX2和CSOX1 gene的抑制作用的抑制作用。在消化器官的器官发生中的CSOX2和CSOX21被证明。此外，积累了用于进一步分析其功能的基本数据。