Evolution and Diversity in the system of development and differentiation

发育和分化系统中的进化和多样性

基本信息

批准号：
12202005
负责人：
GOJOBORI Takashi
金额：
$ 97.92万
依托单位：
National Institute of Genetics
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2004
项目状态：
已结题

项目摘要

(1) Evolution and Developmental mechanism in Nervous systemTo understand when and how the structural brain emerged, we analyzed the central nervous system of a lower invertebrate, planarian. We conducted a large-scale screening of the head part-specific genes in the planarian by constructing a cDNA microarray. As a result, 205 genes with head part-specific spikes were found, which included some essential genes in the vertebrate nervous system. The expression patterns of the top 30 genes showing the strongest spikes implied that the planarian brain has undergone functional regionalization.As a result of homology searches, we found that 116 clones had significant similarity to known genes related to the nervous system. Interestingly, we found that 〜30% of planarian nervous system-related genes had homologous sequences in Arabidopsis and yeast, which do not possess a nervous system. This implies that the origin of nervous system-related genes greatly predated the emergence of the nervous system, and that these genes might have been recruited toward the nervous system.(2) Evolution and Diversity in the system of development and differentiationTo investigate the diversification of cichlid jaw morphology at the genetic level, we used a DNA chip to compare gene expression levels between closely related cichlid fishes. This analysis indicated that the expression of some genes differed in the larvae of two cichlid species. One such clone encodes a new astacin family metalloproteinase. Because some astacin family proteins contribute to morphogenesis in animals, CiMP1 is expected to participate in species-specific head morphogenesis in cichlids.In Cichlids, here, we provide an example in which alternative splicing may play a role in mate choice, leading to cichlid speciation through diversification of gene function by production of multiple mRNAs from a single gene.

(1)神经系统的进化和发育机制为了了解结构性大脑何时以及如何出现，我们分析了低等无脊椎动物涡虫的中枢神经系统，我们通过以下方法对涡虫头部特异性基因进行了大规模筛选。构建了 cDNA 微阵列，结果发现了 205 个具有头部特异性尖峰的基因，其中包括脊椎动物神经系统中的一些必需基因，显示最强尖峰的前 30 个基因的表达模式。暗示涡虫大脑已经经历了功能区域化。通过同源搜索，我们发现 116 个克隆与已知的神经系统相关基因具有显着相似性，这意味着我们发现大约 30% 的涡虫神经系统相关基因。在不具有神经系统的拟南芥和酵母中具有同源序列，这意味着神经系统相关基因的起源大大早于神经系统的出现，并且这些基因可能已被招募到神经系统中。 2）发育和分化系统的进化和多样性为了在基因水平上实现慈鲷颌骨形态的多样化，我们使用DNA芯片来比较研究密切相关的慈鲷鱼之间的基因表达水平。该分析表明，一些基因的表达在不同物种中存在差异。两个丽鱼科鱼种的幼虫。其中一个克隆编码一种新的虾红素家族金属蛋白酶，因为一些虾红素家族蛋白有助于动物的形态发生，因此 CiMP1 有望参与物种特异性头部。慈鲷的形态发生。在慈鲷中，我们在这里提供了一个例子，其中选择性剪接可能在配偶选择中发挥作用，通过从单个基因产生多个 mRNA 来实现基因功能的多样化，从而导致慈鲷的物种形成。

项目成果

期刊论文数量（334）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Pigment cell lineage-specific expression activity of the ascidian tyrosinase-related gene.

DOI：
10.1016/j.gene.2004.01.030
发表时间：
2004-05
期刊：
Gene
影响因子：
3.5
作者：
R. Toyoda;A. Kasai;Shigeru Sato;S. Wada;H. Saiga;K. Ikeo;T. Gojobori;T. Numakunai;Hiroaki Yamamoto
通讯作者：
R. Toyoda;A. Kasai;Shigeru Sato;S. Wada;H. Saiga;K. Ikeo;T. Gojobori;T. Numakunai;Hiroaki Yamamoto

Integrative annotation of 21,037 human genes validated by full-length cDNA clones.

DOI：
10.1371/journal.pbio.0020162
发表时间：
2004-06
期刊：
PLoS biology
影响因子：
9.8
作者：
Imanishi T;Itoh T;Suzuki Y;O'Donovan C;Fukuchi S;Koyanagi KO;Barrero RA;Tamura T;Yamaguchi-Kabata Y;Tanino M;Yura K;Miyazaki S;Ikeo K;Homma K;Kasprzyk A;Nishikawa T;Hirakawa M;Thierry-Mieg J;Thierry-Mieg D;Ashurst J;Jia L;Nakao M;Thomas MA;Mulder N;Karavidopoulou Y;Jin L;Kim S;Yasuda T;Lenhard B;Eveno E;Suzuki Y;Yamasaki C;Takeda J;Gough C;Hilton P;Fujii Y;Sakai H;Tanaka S;Amid C;Bellgard M;Bonaldo Mde F;Bono H;Bromberg SK;Brookes AJ;Bruford E;Carninci P;Chelala C;Couillault C;de Souza SJ;Debily MA;Devignes MD;Dubchak I;Endo T;Estreicher A;Eyras E;Fukami-Kobayashi K;Gopinath GR;Graudens E;Hahn Y;Han M;Han ZG;Hanada K;Hanaoka H;Harada E;Hashimoto K;Hinz U;Hirai M;Hishiki T;Hopkinson I;Imbeaud S;Inoko H;Kanapin A;Kaneko Y;Kasukawa T;Kelso J;Kersey P;Kikuno R;Kimura K;Korn B;Kuryshev V;Makalowska I;Makino T;Mano S;Mariage-Samson R;Mashima J;Matsuda H;Mewes HW;Minoshima S;Nagai K;Nagasaki H;Nagata N;Nigam R;Ogasawara O;Ohara O;Ohtsubo M;Okada N;Okido T;Oota S;Ota M;Ota T;Otsuki T;Piatier-Tonneau D;Poustka A;Ren SX;Saitou N;Sakai K;Sakamoto S;Sakate R;Schupp I;Servant F;Sherry S;Shiba R;Shimizu N;Shimoyama M;Simpson AJ;Soares B;Steward C;Suwa M;Suzuki M;Takahashi A;Tamiya G;Tanaka H;Taylor T;Terwilliger JD;Unneberg P;Veeramachaneni V;Watanabe S;Wilming L;Yasuda N;Yoo HS;Stodolsky M;Makalowski W;Go M;Nakai K;Takagi T;Kanehisa M;Sakaki Y;Quackenbush J;Okazaki Y;Hayashizaki Y;Hide W;Chakraborty R;Nishikawa K;Sugawara H;Tateno Y;Chen Z;Oishi M;Tonellato P;Apweiler R;Okubo K;Wagner L;Wiemann S;Strausberg RL;Isogai T;Auffray C;Nomura N;Gojobori T;Sugano S
通讯作者：
Sugano S

Evolution of metabolic networks by gain and loss of enzymatic in eukaryotes.

真核生物中酶的获得和损失导致代谢网络的进化。